Suppose you want to study the transcription in vitro of one particular gene in a DNA molecule that contains several genes and promoters. Without adding specific regulatory proteins, how might you stimulate transcription from the gene of interest relative to the transcription of the other genes on your DNA template? To make all of the complexes identical, you would like to arrest all transcriptional events at the same position on the DNA template before isolating the complex. How might you do this?

Question

Suppose you want to study the transcription in vitro of one particular gene in a DNA molecule that contains several genes and promoters. Without adding specific regulatory proteins, how might you stimulate transcription from the gene of interest relative to the transcription of the other genes on your DNA template? To make all of the complexes identical, you would like to arrest all transcriptional events at the same position on the DNA template before isolating the complex. How might you do this?

Expert Answer

Want to see the step-by-step answer?

Check out a sample Q&A here.

Want to see this answer and more?

Experts are waiting 24/7 to provide step-by-step solutions in as fast as 30 minutes!*

*Response times may vary by subject and question complexity. Median response time is 34 minutes for paid subscribers and may be longer for promotional offers.
Tagged in
Science
Biochemistry

Gene expressions

Related Biochemistry Q&A

Find answers to questions asked by students like you.

Q: If a 0.2g of oil consumed 1ml of sodium thiosulphate, calculate its Iodine value and classify the oi...

A: The iodine value in science is the mass of iodine in grams that is devoured by 100 grams of a synthe...

Q: Suggest a name for an enzyme that catalyzes each of t a.) Oxidation of nitrite b.) Decarboxylation o...

A: An enzyme is a substance that acts as a catalyst in living organisms, regulating the rate at which c...

Q: During glycogen synthesis in vertebrates, _____ is added to the growing chain by glycogen synthase. ...

A: Glycogen is considered as a storage material for the animals. This helps in the production of energy...

Q: what is the function of Pyruvate carboxylase, Alcohol dehydrogenase

A: Introduction: The major source of energy for all the living cells on this planet is carbohydrates. I...

Q: The tricarboxylate transport system supplies cytosolic acetyl-CoA for palmitate synthesis. What perc...

A: The biosynthesis of plamitate requires acetyl-CoA and also the input of chemical energy in two forms...

Q: Biochemistry Question

A: Introduction: Molality is the ratio of the number of moles of solute per kilogram of solvent. Its SI...

Q: Oil is a major energy-storage molecule in most seeds. During seed development in some plants, oil (t...

A: Seed refers to an embryonic plant enclosed in a protective outer covering. They are the products of ...

Q: 1. For each of the processes below, fill in blanks with one or more of the items in the following li...

A: “Since you have asked multiple questions, we will solve the first question for you. If you want any ...

Q: Some anaerobic prokaryotes reduce elemental sulfur to H2S. Assuming 100% efficiency, how much ATP co...

A: Anaerobic respiration is the conversion of sugar into energy in absence of oxygen. This kind of resp...

Q: Please help with these quickly.

A: Dynamic instability is the condition, which is possible because of GTP hydrolysis. GTP distorted the...

Q: Niacin is involved in the TCA cycle or ETC as:

A: Niacin is considered Vitamin B3, which is an important chemical signal. Moreover, it acts as an acti...

Q: The size and shape of the atomic orbitals have many important implications for bonding and intermole...

A: Angular nodes are flat planes, ones that are found at at fixed angles. The quantum number l determin...

Q: Herceptin is an antibody that is used to treat certain forms of breast cancerby binding to a class o...

A: Herceptin is marketed under the name of trastuzumab(approved by US Food and Drug Administration), wh...

Q: Lactose (and also maltose) hydrolyzes upon prolonged heating and gives positive result with Seliwano...

A: Seliwanoff's test is used to differentiate between ketohexose and aldohexose. Ketoheoxose- Functiona...

Q: For the following short sequence of double stranded DNA, design primers (just ~ 3-4 bases) and show ...

A: Polymerase (PCR) chain reaction is a process in which small DNA fragments are amplified into a billi...

Q: Consider the pathway for catabolizing galactose. What are the potential control points for this path...

A: Galactose is metabolized mainly in the liver, which is an important dietary sugar. Lactose gives gal...

Q: When you made soap, first you dissolved vegetable oil in ehanol. What happened to the ethanol during...

A: Soaps are sodium or potassium fatty acids (that is long carbon chain) salts, produced from the hydro...

Q: Please explain the differences between 100% glucose and 0% glucose for all four processes: Glycolysi...

A: Cellular aerobic respiration is conversion of chemical energy in the form of Glucose into ATP which ...

Q: Why is TBP considered to be a universal transcription factor?

A: The TATA-binding protein is a general transcription factor that binds specifically to a DNA sequence...

Q: The saponifiable /nonsaponifiable classification system for lipid is based on

A: Lipids are the organic compounds, which are fatty acids or the derivatives of fatty acids. Lipids ar...

Q: Adenylate cyclase, which synthesizes cyclic AMP from ATP, requires two metal ions, and the enzyme ha...

A: Adenosine triphosphate (ATP) is an energy carrying molecule found in the cells of all living things....

Q: Write a report on the chemistry and modes of action of the following mitochondria poisons? Rotenone...

A: Information about respiratory chain has been gained by the use of site-specific inhibitors of electr...

Q: Describe the chemical difference between mannose and heptyl alpha-D-mannose. Why does FimH have a hi...

A: Mannose is a type of the monomer of the sugar. Mannose can be produced in the human body and it can ...

Q: Which of the mechanisms for dissipating light energy shown in Fig. would best protect the photosyste...

A: For plant survival and fitness, effective photosynthesis is of fundamental importance. However, the ...

Q: Please help with these quickly.

A: Cell migration is an important process, both in unicellular organisms such as amoeba and in highly e...

Q: What metal is present in ALL complexes of the electron-transport chain? O a. potassium O b. manganes...

A: Since you have asked multiple questions, we will solve the first question for you. If you want any s...

Q: There is evidence that some oxidative damage to DNA occurs at the nucleotide level, with oxidation o...

A: Deoxyribonucleic acid (DNA) is a molecule composed of two polynucleotide chains. It coils around eac...

Q: Explain two hypothetical ways an active transposon could cause trouble for an organism.

A: DNA transposons are DNA sequences, sometimes referred to "jumping genes", that can move and integrat...

Q: Explain why electron transport in purple photosynthetic bacteria follows a circular path.

A: Electron transport during photosynthesis can be either cyclic or non-cyclic in order to synthesis AT...

Q: What is the significance of the coagulation of egg white to its digestion? Why is alcohol an effecti...

A: Coagulation is the process through which the structure of the protein is changed by the action of ac...

Q: A nonsense mutation is a substitution mutation that creates a chain-terminating codon in the mRNA co...

A: Mutation is the sudden heritable changes that occur in the DNA sequences due to error while copying ...

Q: Laboratory Report Sheet – ENZYMES Procedure: Potato Peroxidase a. Prepare 3 test tubes. Place 1 mL ...

A: Catalase is an enzyme that acts as a biocatalyst. It is widely available in the peroxisomes of plant...

Q: When enzyme solutions are heated, there is a progessive loss of catalytic activty over time due to d...

A: Enzyme is defined as a biological catalyst that speeds up the rate of chemical reaction. All biologi...

Q: Enzyme + acetate + CoA ---> enzyme + acetyl _ CoAIn the reaction above, the enzyme is acting as a...

A: Enzymes are composed of proteins, which perform several functions as an activator, modulators, cofac...

Q: Fermentation will occur under anaerobic conditions only Sometimes the condition is temporary, result...

A: The metabolism is a process, during which energy is produced in the form of ATP, NADH, and others. I...

Q: 1.What is the chemic name and formula of the precipitate for the test for phosphate? 2.suppose biure...

A: Dear students, As the given question has multiple questions and here it is not specified which ques...