Answered: Suspend 28.0 grams in the 1000 mL… | bartleby

Complete Textbook Of Phlebotomy

Complete Textbook Of Phlebotomy

5th Edition

ISBN:9781337464314

Author:Hoeltke

Publisher:Hoeltke

Chapter10: Sample Preparation And Handling

Section: Chapter Questions

Problem 4CT

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Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (1210C) for 15 minutes. Cool to 45-500C. If desired the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri dishes.

1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses.

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