Procedure A stock solution of 100 mg dm3 gibberellic acid should be prepared. Make a series of dilutions to give a range of concentrations of 10 mg dm3, 1.0 mg dm 3, 0.1 mg dm, and 0.01 mg dm3. Place a piece of filter paper in each of the six Petri dishes. Pipette 5 cm of each of the gibberellic acid solutions, one sample into each of five of the dishes. Into the sixth, pipette 5 cm of distilled water. In each dish place 20 germinating lettuce seedlings with radicles 5 mm long. Leave the dishes in diffuse light for a period of 4-5 days. At the end of this time measure the lengths of the hypocotyls. During the 4-5 days the solutions may lose water by evaporation; this should be compensated for. preliminary ran with water in a petri dish containing a filter paper will allow a fairly close estimate of evaporation rates. Plot a graph of hypocotyl growth against the logarithm of GA concentration.

Procedure A stock solution of 100 mg dm3 gibberellic acid should be prepared. Make a series of dilutions to give a range of concentrations of 10 mg dm3, 1.0 mg dm 3, 0.1 mg dm, and 0.01 mg dm3. Place a piece of filter paper in each of the six Petri dishes. Pipette 5 cm of each of the gibberellic acid solutions, one sample into each of five of the dishes. Into the sixth, pipette 5 cm of distilled water. In each dish place 20 germinating lettuce seedlings with radicles 5 mm long. Leave the dishes in diffuse light for a period of 4-5 days. At the end of this time measure the lengths of the hypocotyls. During the 4-5 days the solutions may lose water by evaporation; this should be compensated for. preliminary ran with water in a petri dish containing a filter paper will allow a fairly close estimate of evaporation rates. Plot a graph of hypocotyl growth against the logarithm of GA concentration.

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

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