The barbituate drug amytal inhibits complex I from passing electrons to Coenzyme Q. Circle your choice for each pair of options, if amytal is present. 9. a. Do you expect the mitochondria to contain NADH + H* -or- NAD ? FADH, -or- FAD? b. What is the expected redox state (oxidized "Ox" or reduced "Red") for complexes I-IV of ETC? i. Complex I Ox -or- Red iii. Complex IIl Ox -or- Red ii. Complex Il Ox -or- Red iv. Complex IV Ox -or- Red c. Will ATP be produced? Yes -or- No d. Is ETC/OxPhos uncoupled? Yes -or- No e. Will a proton gradient be present? Yes -or- No I

The barbituate drug amytal inhibits complex I from passing electrons to Coenzyme Q. Circle your choice for each pair of options, if amytal is present. 9. a. Do you expect the mitochondria to contain NADH + H* -or- NAD ? FADH, -or- FAD? b. What is the expected redox state (oxidized "Ox" or reduced "Red") for complexes I-IV of ETC? i. Complex I Ox -or- Red iii. Complex IIl Ox -or- Red ii. Complex Il Ox -or- Red iv. Complex IV Ox -or- Red c. Will ATP be produced? Yes -or- No d. Is ETC/OxPhos uncoupled? Yes -or- No e. Will a proton gradient be present? Yes -or- No I

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter18: Glycolysis

Section: Chapter Questions

Problem 12P

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10) The phosphorylation of glucose is an unfavorable reaction, with ΔG°’ = 13.8 kJ/mol. We can couple this to the favorable reaction of ATP hydrolysis to make the overall process favorable. a) Describe how this works. b) If ΔG°’ for ATP hydrolysis = -32.2 kJ/mol, what is the overall ΔG°’ of the reaction?
4. If lots of acetyl-CoA is being formed in the mitochondrial matrix, a particular compound will be exported into the cytosol. a.What compound is this? b. What effect does this compound have on fatty acid synthesis? c. How does the compound produce this effect?
1. A cell in an active catabolic steady state state needs to maintain A. a high cytosolic (ATP/ADP) and a high cytosolic (NADH/NAD+) ratio. B. a high cytosolic (ATP/ADP) and a low cytosolic (NADH/NAD+) ratio. C. a low cytosolic (ATP/ADP) and a low cytosolic (NADH/NAD+) ratio. D. a low cytosolic (ATP/ADP) and a high cytosolic (NADH/NAD+) ratio.
5. a) The cell creates molecules of NADH and FADH2to use in the electron transport chain as they are electron carrier molecules. The electron transfers from these moleculesdrives the movement of what molecule across the mitochondrial membrane?b) What is chemiosmosis?c) How does the cell use chemiosmosis to drive overcome the thermodynamic barrier of bringing phosphates close togehter to create ATP?d) What steps of oxidative phosphorylation are in the cytosol and which are in the mitochondria?e) Cellular respiration can be controlled at the post-translational level. What does this mean, using GLUT as an example? Why is post-translational control of GLUT evolutionarilyadaptive over transcriptional control?f) How does establishment of equillibria of various molecules control metabolic reactions? What are three ways high ATP in the cell control cellular respiration?g) Why are the enzymes controlling the initial steps of various biochemical pathways are targeted by allosteric control?
1) When a de-coupler such as 2,4-DNP is added to a respiring mitochondrion, which of the following effects on the process of ETC-OP will be true, which will be false. a) Mitochondria no longer reduce O2 to H2 b) Electrons no longer enter the electron transport chain. c) Activity of Complex V (ATP Synthase) is greatly decreased. d) The P/O ratio for FADH2 becomes larger than the P/O ratio for NADH. e) Protons are no longer pumped from the mitochondrial matrix to the intermembrane space. 2) The rate-limiting step in fatty acid synthesis is: a) condensation of acetyl-CoA and malonyl-CoA. b) formation of acetyl-CoA from acetate. c) formation of malonyl-CoA from malonate and coenzyme A. d) the reaction catalyzed by acetyl-CoA carboxylase.
1 a) What is meant by the ATP currency exchange ratio? Why does the oxidation of mitochondrial FADH2 generate one less ATP than oxidation of mitochondrial NADH? b) If 12 H+ are moved across the inner mitochondrial membrane by NADH oxidation, and each ATP synthesized requires 3 H+ to move through ATP synthase, why are only 3 ATP molecules produced by oxidation of each NADH?
1. For the complete oxidation of 16 glucose molecules as a result of cellular respiration in plants, how many of the following would be produced? Show your computation. A. Acetyl Co-A B. Total NADH C. Total FADH2 D. Total ATP E. Total GTP
1. Identify the oxidized coenzyme (letter abbreviation only) that participates in this reaction of the Kreb’s cycle. Succinate --> Fumarate 2. How many mol of NADH can be obtained upon the beta oxidation of stearic acid? 3. How many mol of ATP can be obtained upon the complete oxidation of 1 mol stearic acid? 4. How many steps in glycolysis in which ATO is converted to ADP?
8. Compare the ATP yield for the complete oxidation of glutamate and methionine, both of which contain fi ve carbon atoms, to CO2. you can assume NADH (or NADPH) and FADH2 yield 2.5 and 1.5 ATP equivalents, respectively. The oxidation of malate is performed by malic enzyme
1. Why is the chemical logic behind the phosphorylation of glucose by hexokinase? a. So that we end the preparatory phase with 2 3-carbon compounds. b. to provide a substrate for phosphohexose isomerase. c. "Locks" Glucose in the cell. d. to decrease the ΔG of the next reaction. 2. What type of reaction does glyceraldehyde-3-phosphate dehydrogenase catalyze? a. isomerization b. group transfer c. oxidation d. dehydration
7. Why are electron carriers (NAD+/NADH and FAD/FADH2) so important in the process of cellular respiration? a) They deliver electrons to the ETC, which in turn sets up chemiosmosis, where most of the ATP is generated. b) They separate the electrons from the protons so that the protons can be moved out of the mitochondrion. c) NADH and FADH2 are major components of the ETC, so without them, there would be no ETC in the cell. d) The electrons that they carry are able to directly phosphorylate ADP in order to generate the bulk of ATP in the cell. e) They transport protons across the mitochondrial membrane.
9 .The energy of electron transport (by the ETC) serves to move protons (hydrogen ions, or H+) to the intermembrane space of the mitochondrion. How does this help the mitochondrion produce ATP? a) This movement of protons sets up the electrochemical gradient that drives ATP synthesis in the mitochondrion. b) The protons pick up electrons from the electron transport chain on their way through the inner mitochondrial membrane c) The protons receive electrons from the NAD+ and FAD that were accepted in glycolysis ad the citric acid cycle d) The protons are transferred to oxygen in an energy-releasing reaction