The BSA stock solution from the previous problem was then diluted to generate a set of standard solutions of known concentrations. After performing biuret assay on these solutions, their absorbance at 540 nm were measured using a UV-vis spectrophotometer. The following data were obtained. Concetration of BSA Absorbance (mg/mL) 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8 0.380 1.0 0.485 Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.
The BSA stock solution from the previous problem was then diluted to generate a set of standard solutions of known concentrations. After performing biuret assay on these solutions, their absorbance at 540 nm were measured using a UV-vis spectrophotometer. The following data were obtained. Concetration of BSA Absorbance (mg/mL) 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8 0.380 1.0 0.485 Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 7.2P
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