Sugar to Glycolysis Madeupose-5 Phosphate (a 5 carbon aldose) is fed into glycolysis after a carboxylation reaction that uses an NADH to generate a 6 carbon ketose. How many ATP and NADH are generated by breaking down this molecule to pyruvate?
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- The Effect of lodoacetic Acid on the Glyceraldehyde-3-P Dehydrogenase Reaction (Integrates with Chapters 4 and 14.) How might iodoacetic acid affect the glyceraldehydes-3-phosphate dehydrogenase reaction in glycolysis? Justify your answer.Comparing Glycolysis Entry Points for Sucrose Sucrose can enter glycolysis by either of two routes: Sucrose phosphorylase: Sucrose + Pi fructose + glucose-1-phosphate Invertase: Sucrose + H20 fructose + glucose Would either of these reactions offer Jin advantage over the other In the preparation of hexoses fur entry into glycolysis?The Reactions and Meehanisms of the Leloir Pathway Write the reactions that permit galactose to be utilized in glycolysis. Write a suitable mechanism, tor one of these reactions.
- Ethanol as a Source of Metabolic Energy (Integrates with Chapters 19 and 20.) Acetate produced in ethanol metabolism can be transformed into acetyl-COA by the acetyl thiokinase reaction: Acetate+ATP+CoASHacetyleCoA+AMP+PPiAcetyle-CoA then can enter the citric acid cycle and undergo oxidation to 2 CO2by this route, assuming oxidative phosphorylation is part of the process? (Assume all reactions prior to acetyl-CoA entering the citric acid cycle occur outside the mitochondrion). Per carbon atom, which is a better metabolic fuel, ethanol or glucose? That is, how many ATP equivalents per carbon atom are generated by combustion of glucose versus ethanol to CO2?Understanding Enzyme Mechanisms Related to Pyruvate Carboxylase Based on the mechanism for pyruvate carboxylase (Figure 22.3), write reasonable mechanisms for the reactions that follow:Understanding the Oxidation of Glucose and Its Products in the TCA Cycle Glycolysis, the pyruvate dehydrogenase reaction, and the TCA cycle result in complete oxidation of a molecule of glucose to CO2. Review the calculation of oxidation numbers for individual atoms in any molecule, and then calculate the oxidation numbers of the carbons of glucose, pyruvate, the acetyl carbons of acetyl-CoA. and the metabolites of the TCA cycle to convince yourself that complete oxidation of glucose involves removal of 24 electrons and that each acetyl-CoA through the TCA cycle gives up 8 electrons.
- threose-4 phosphate (a 4 carbon aldose) is fed into glycolysis after a decarboxylation reaction that uses an NAD+ to generate a 3 carbon aldose. How many ATP and NADH are generated by breaking down this molecule to pyruvate?Thioester play an important role in glycolysis and the TCA cycle. Which reactions in glycolysis and tca cycle involve thioesters.Glucose is converted to pyruvate in glycolysis, yielding a netsynthesis of 2 ATP. In certain cells pyruvate can be reconverted to glucose during gluconeogenesis. How many ATPsare required to convert pyruvate back to glucose?
- How many molecules of ATP are produced from the complete metabolism of 2.12g of triacylglycerol containing two stearic acid chains and one palmitic acid chain? Assume that all electrons of cytosolic NADH are transferred through the dihydroxyacetone phosphate/glycerol 3-phosphate shuttleFatty acid degradation stimulates the citric acid cyclethrough the activation of pyruvate carboxylase by acetylCoA. Why would the activation of pyruvate carboxylaseincrease energy generation from fatty acids?the first two reactions in glycolysis associated with unfavorable ∆G° values, i.e., ∆G° > 0, both produce a product with high phosphoryl group transfer potential. (ATP formation is not considered here.) Compare the two reactions and describe what the driving force is for both reactions that allows glycolysis to proceed to formation of pyruvate.