The end products of protein metabolism is often ammonia, a base. What effect will this have on pH, will it go up or down? Another common end product, pyruvic acid. Into which metabolic pathway will the body use pyruvic asid as a substrate to produce ATP?

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The end products of protein metabolism is often ammonia, a base. What effect will this have on pH, will it go up or down?

Another common end product, pyruvic acid. Into which metabolic pathway will the body use pyruvic asid as a substrate to produce ATP?

Lab time
Prelab 7 - Protein Catabolism
In this lab we focus on another important group of metabolism processes: the breakdown of proteins and
amino acids. In these labs we will utilize a series of media in tubes that differ in the protein for the bacteria to
breakdown, and in the end products they can detect. There are a few new vocabulary terms. Proteolytic
enzymes are enzymes that breakdown proteins, usually by first breaking apart the polypeptide into individual
amino acids. Hydrolysis reaction is used to break apart the peptide bond holding the amino acids in the
polypeptide together. Hydrolysis is used to breakdown urea, a compound produced by the body from the
destruction of proteins.
Once broken down to individual amino acid, often the amine (NH2) functional group is broken off one end in a
deamination reaction, creating NH3. In a decarboxylation reaction the carboxyl group is removed, creating
CO2. In a desulfuration reaction a sulfur is removed. Examples of these processes can be found in figures in
the lab background information.
1. The end product of protein metabolism is often ammonia, a base. What effect will this have on pH, will it go
up or down?
2. Another common end product, pyruvic acid. Into which metabolic pathway will the body use pyruvic acid as
a substrate to produce ATP?
To become familiar with the media before lab, please read both protein catabolism exercise background
information. In particular pay attention to the figures. Similar to last lab, the table below summarizes the
different metabolic tests. Using the table, interpret the results in the following questions. There are results to
interpret on the back of this handout.
D, Focus
Transcribed Image Text:Lab time Prelab 7 - Protein Catabolism In this lab we focus on another important group of metabolism processes: the breakdown of proteins and amino acids. In these labs we will utilize a series of media in tubes that differ in the protein for the bacteria to breakdown, and in the end products they can detect. There are a few new vocabulary terms. Proteolytic enzymes are enzymes that breakdown proteins, usually by first breaking apart the polypeptide into individual amino acids. Hydrolysis reaction is used to break apart the peptide bond holding the amino acids in the polypeptide together. Hydrolysis is used to breakdown urea, a compound produced by the body from the destruction of proteins. Once broken down to individual amino acid, often the amine (NH2) functional group is broken off one end in a deamination reaction, creating NH3. In a decarboxylation reaction the carboxyl group is removed, creating CO2. In a desulfuration reaction a sulfur is removed. Examples of these processes can be found in figures in the lab background information. 1. The end product of protein metabolism is often ammonia, a base. What effect will this have on pH, will it go up or down? 2. Another common end product, pyruvic acid. Into which metabolic pathway will the body use pyruvic acid as a substrate to produce ATP? To become familiar with the media before lab, please read both protein catabolism exercise background information. In particular pay attention to the figures. Similar to last lab, the table below summarizes the different metabolic tests. Using the table, interpret the results in the following questions. There are results to interpret on the back of this handout. D, Focus
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