If any of the water sample plates had a lawn or too many colonies to count, what might you have done, without reducing the volume of liquid you added to the plate, so that you had ended with a countable number of colonies?
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If any of the water sample plates had a lawn or too many colonies to count, what might you have done, without reducing the volume of liquid you added to the plate, so that you had ended with a countable number of colonies?
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- Did each handwashing treatment result in similar types of organisms growing on the plates? (look at the colonies and gram types) Explain why or why not?What do to do when the disstillation did not make the sample completely pure? What could have went wrong?You make a 1:100 dilution of your culture and plated 0.1 ml of this sample on a Nutrient agar plate. After incubation, you counted 50 colonies on the plate. How many organisms were in the original culture?
- pless dont proved hand ratting solutionIf 1.0 mL of a 1:1000 dilution produces 200 colonies on a culture plate, how many bacteria were present per mL in the original (undiluted) sample? 200,000 20,000 2,000 200From a water sample, you must determine the number of colonies present (CFU's/ml.). However, looking at the plates of the dilutions you find some cultures with scattered colonies. Briefly explain the reason for eliminating or not considering these plates in the data collection. Short answer please..
- Why should your solvent be evaporated from the sample placed on the sterile disk before you place it your petri dish?A 2x10-6 dilution of your bacterial culture yielded 10 colonies. What was the cell density (incells/ml) of your bacterial culture?On agar plate does each discrete colony represent the growth of one cell? Explain your answer. Why can a single colony on a plate be used to start a pure culture?
- Match the samples to the type pf solution they belong.Three grams of soil from the ground were added to 27 mL of sterile water and shaken vigorously. After the soil particles settled, 0.1 mL of this was added to 9.9 mL of sterile water. This was further diluted by 4 successive 1/10 dilutions. One mL from the last dilution was used to prepare a pour plate. After incubation, 289 colonies were present on this plate. What was the number of colony-forming units/gram of the soil?Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram stain on a sample before prescribing an antibiotic?