What side of the electrophoresis chamber (red or black) should the wells be on when the gel is placed in the chamber? What would happen if you placed the agarose gel with the wells on the opposite side of the chamber?

Q: Why is the order of separation based on size opposite for gel filtration and gel electrophoresis,…

A: Gel filtration and gel electrophoresis are biochemical techniques used for the separation of…

Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…

A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…

Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…

A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…

Q: how do you purify a protein for single particle cryo-electron microscopy?

A: We would require a protein sample with more than 99% purity in order for it to qualify as a sample…

Q: Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer

A: Disclaimer: “Since you have asked multiple questions, we will solve the first question for you. If…

Q: The gelatin medium required inoculation with 3 loopfuls of culture. Why was so much organism…

A: Gelatin hydrolysis test is carried for detecting the ability of the bacteria to produce protease…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside…

A: Several laboratory techniques necessitate smear preparation. A smear can be made using either broth…

Q: How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of…

A: Dilution is the process of decreasing the concentration of a solute in a solution. To dilute a…

Q: to observe the motility of an UNKNOWN Bacteria isolated from a mixed culture, which technique/s is…

A: Motility is the ability of an organism to move from one place to other, generally, the bacteria…

Q: A powdered complex medium can be stored for months in the lab. However, after rehydrating the medium…

A:

Q: Which of the following cannot be grown in artificial media and must be grown in live animals or…

A: Artificial media is a medium that is defined. This medium is chemically defined. It is a synthetic…

Q: Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) 1. Why do you destain the gel?

A: There are various electrophoresis techniques used for the separation and identification of proteins.…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is…

A: The following are needed for calculating the dilution factor  1) Original volume of  solution we…

Q: Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can…

A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…

Q: Identify the following on the gel electrophoresis 2-leg ladder 3 Kb 1022 bp 1022 bp 880 bp 765 hp…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…

A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…

Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…

A: Given data:  Each plate contains-  LB agar=20ml Ampicillin= 150 micrograms per ml.  Amount of…

Q: What is the color result of 1% solution of casein, Albumin, Gelatin in ninhydrin test? Include…

A: Ninhydrin test: Chemical test to detect presence of ammonia, primary/secondary amines, or amino…

Q: During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture…

A: Capsule stain is a type of differential stain which uses acidic and basic dyes to stain the…

Q: A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture…

A: A growth medium is intended to promote the growth of a variety of microorganisms or cells by the…

Q: gel electrophoresis

A: Gel Electrophoresis is a simple , rapid and analytical technique which is used for separating the…

Q: In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable…

A: Harada-Mori culture technique It is a method of incubating fecal material on a filter paper strip in…

Q: How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the…

A: Serial dilution is used mostly in studies involving cell cultures (microbiology or even cell culture…

Q: In a polyacrylamide gel electrophoresis, the gel consists of specific gel components. name them

A: A type of gel electrophoresis (GE) that is used to quantify and separate desired protein molecules…

Q: Solid media is usually prepared with a 1-2% final concentration of agar. If these plates were…

A: Solid media is the type of media that is made up of liquid substances like distilled water and other…

Q: For a TLC experiment, what is the purpose of the pentane extraction of spinach juice? Why wouldn’t…

A: Answer: Introduction:Thin layer chromatography, or TLC, is an analytical method to analyse mixtures…

Q: What is native PAGE used for?

A: Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…

A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…

Q: According to these images and your own knowledge, can you make a biological drawing of bacteria in…

A: Yoghurt is a nutrient which is rich in lactobacillus (lactic acid bacteria) . Convert milk into…

Q: If there is 50 bacterial colonies on a 1:1000 dilution streak plate. How many cfu/mL are there?

A: Introduction: A CFU stands for colony-forming units. It is a unit that we use for estimating the…

Q: In the preparation of a pure culture media, the petri plates are inverted when placed in an…

A: In microbiology, culture media is considered the growth medium that possesses the specific mixtures…

Q: If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock…

A: Given: volume of stock solution is 15 mg/100 ml Needed amount is 1 mg kinetin in 25 ml Volume of…

Q: Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium…

A: Bacteria are organisms that are found almost everywhere on earth and are free-living cells that are…

Q: Which of the following samples are expected to cause a decrease in absorbance over time when…

A: Ensuring adequate light reaching a detector by using water colloidal solutions in light or X-ray…

Q: How many bacteria would be in 1ml of the sample if you counted 134 colonies on the 1:1,000 dilution?

A:

Q: What types of compounds make up the gels used in electrophoresis?

A: The types of macromolecules usually separated on Agarose gel are : Nucleic acids-mainly DNA…

Q: In Gram staining method, list the reagents and state their role for each step of the procedure? In…

A: Bacteria are unicellular, prokaryotic organicism which are devoid of membrane bound organelles and a…

Q: When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies…

A: Dilution is the process of decreasing the concentration of the solution. The cells count decreases…

Q: Which of the following is/are true regarding the acid-fast stain? (There may be more than one…

A: Answer  First option  Non-acid-fast microbes appear blue in a completed acid-fast stain.

Q: You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is…

A: The dilution factor may be expressed as the ratio of the concentration of stock solution to the…

Q: Discuss the functions of the following components in performing gel electrophoresis;…

A: Introduction: The technique of gel electrophoresis is used to separate DNA fragments based on their…

Q: You dilute 1 mL of bacterial culture with 99 mL of water.  What is the resulting dilution factor?

A: Dilution factor:In order to calculate the Dilution factor, the final volume is to be divided by the…

Q: The DNA fragments separated on an agarose gel can be visualised after staining with what?

A: Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis…

Q: In preparing bacterial smear, why do we need to pass the slide over the flame?

A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…

Q: In RPR test, Why is it necessary to rotate the slide/card?

A: RPR stands for Rapid Plasma Reagin. It is done to detect syphilis.

Q: Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you…

A: Culture consists of appropriate substances which provides the growth to the desired organisms.

Q: If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a…

A: Serial dilution  It is a method of stepwise dilution of a concentrated solution to get the desired…