Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
The molecular weight of an unspecified protein, is 70,000 Dalton, as determined by gel filtration chromatography. The SDS-polyacrylamide gel electrophoresis (SDS PAGE) of the protein yields a single band corresponding to molecular weight of 70,000 Dalton. However, in the presence of B-mercaptoethanol, the SDS PAGE shows two bands, corresponding to molecular weights of 30,000 and 20,000 Dalton. What is the subunit composition of this protein? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. The protein contains two 30 kD polypeptides and one 20 kD polypeptides. The 20 kD chain is disulfate bonded to a both 30 kD chains. The protein contains 3 subunits, of molecular mass 30 kD, 20 kD, 20 kD, respectively. The subunits are disulfate bonded with each other. The protein contains two 20 kD polypeptides and one 30 kD polypeptides. Each 20 kD chain is noncovalently associated to the 30 kD chain. None of the above.

The molecular weight of an unspecified protein, is 70,000 Dalton, as determined by gel filtration chromatography. The SDS-polyacrylamide gel electrophoresis (SDS PAGE) of the protein yields a single band corresponding to molecular weight of 70,000 Dalton. However, in the presence of B-mercaptoethanol, the SDS PAGE shows two bands, corresponding to molecular weights of 30,000 and 20,000 Dalton. What is the subunit composition of this protein? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. The protein contains two 30 kD polypeptides and one 20 kD polypeptides. The 20 kD chain is disulfate bonded to a both 30 kD chains. The protein contains 3 subunits, of molecular mass 30 kD, 20 kD, 20 kD, respectively. The subunits are disulfate bonded with each other. The protein contains two 20 kD polypeptides and one 30 kD polypeptides. Each 20 kD chain is noncovalently associated to the 30 kD chain. None of the above.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question

Help me

The molecular weight of an unspecified protein, is 70,000 Dalton, as determined by gel filtration chromatography. The SDS-polyacrylamide gel electrophoresis (SDS PAGE) of the protein yields a single band corresponding to molecular weight of 70,000 Dalton. However, in the presence of B-mercaptoethanol, the SDS PAGE shows two bands, corresponding to molecular weights of 30,000 and 20,000 Dalton. What is the subunit composition of this protein? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. The protein contains two 30 kD polypeptides and one 20 kD polypeptides. The 20 kD chain is disulfate bonded to both 30 kD chains. The protein contains 3 subunits, of molecular mass 30 kD, 20 kD, 20 kD, respectively. The subunits are disulfate b. bonded with each other. The protein contains two 20 kD polypeptides and one 30 kD polypeptides. Each 20 kD chain is noncovalently associated to the 30 kD chain. None of the above.
Transcribed Image Text:The molecular weight of an unspecified protein, is 70,000 Dalton, as determined by gel filtration chromatography. The SDS-polyacrylamide gel electrophoresis (SDS PAGE) of the protein yields a single band corresponding to molecular weight of 70,000 Dalton. However, in the presence of B-mercaptoethanol, the SDS PAGE shows two bands, corresponding to molecular weights of 30,000 and 20,000 Dalton. What is the subunit composition of this protein? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. The protein contains two 30 kD polypeptides and one 20 kD polypeptides. The 20 kD chain is disulfate bonded to both 30 kD chains. The protein contains 3 subunits, of molecular mass 30 kD, 20 kD, 20 kD, respectively. The subunits are disulfate b. bonded with each other. The protein contains two 20 kD polypeptides and one 30 kD polypeptides. Each 20 kD chain is noncovalently associated to the 30 kD chain. None of the above.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Digestive system
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS