The rates at various substrate conce are recorded as follows: (b) 2.5 5.0 10.0 15.0 IS]/10 M 2.2 3.8 5.9 7.1 Vo/M min Assuming the reaction obeys Michaelis-Menten kinetics, calculate the following values by using Lineweaver-Burk plot (1) maximum rate, Vmax- (i) Michaelis constant, Km-

The rates at various substrate conce are recorded as follows: (b) 2.5 5.0 10.0 15.0 IS]/10 M 2.2 3.8 5.9 7.1 Vo/M min Assuming the reaction obeys Michaelis-Menten kinetics, calculate the following values by using Lineweaver-Burk plot (1) maximum rate, Vmax- (i) Michaelis constant, Km-

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

The rates at various substrate concentrations for an enzyme-catalysed reaction
(b)
are recorded as follows:
2.5
5.0
10.0
15.0
(S]/10 M
2.2
3.8
5.9
7.1
Vo/ M min
Assuming the reaction obeys Michaelis-Menten kinetics, calculate the following
values by using Lineweaver-Burk plot
()
maximum rate, Vmax-
(i)
Michaelis constant, Km-
(ii)
turnover number, kz.

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