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- For the determination of Pb in blood, a 5.00 mL sample was treated with trichloroacetic acid to precipitate the proteins. After centrifugation, the resulting solution was brought to pH 3 and extracted with two 5 mL portions of methyl isobutyl ketone containing the lead complexing agent APCD (ammonium 1-pyrrolidine carbodithiolate). The extract was drawn directly into an air-acetylene flame which produced an absorbance of 0.444 at 283.3 nm. Two 5.00 mL aliquots of solutions containing 0.250 and 0.450 ppm of Pb were subjected to the same sample treatment and the absorbance that occurred was 0.396 and 0.599 respectively. Calculate the concentration of Pb in µg/dL in the blood.5 optimise, produce and market a commercial assay kit for the enzyme-linked determination of glucose in a range of samplesA protein consists of two types of peptide chains (A and B) with an unknown stoichiometry (AxBy). When you ran this protein directly on reversed phase-HPLC with UV monitor set at 280 nm, two peaks were resolved. Mass spec determined that the peaks represented Chain A and Chain B, respectively. The peak area is 500,000 for Peak A (Chain A) and 100,000 for Peak B (Chain B). The molecular masses of Chain A and Chain B are 25,000 and 5000, respectively. The extinction coefficients for Chain A and Chain B are 1 mL/mg.cm and 0.5 mL/mg.cm, respectively. Please calculate x/y.
- The data below give the concentration of thiol (mM) in the blood lysate of the blood of two groupsof volunteers, the first group being ‘normal’ and the second having rheumatoid arthritis (Banford, J.C.,Brown, D.H., McConnell, A.A., McNeil, C.J., Smith, W.E., Hazelton, R.A. and Sturrock, R.D., 1983,Analyst, 107: 195): Normal: 1.84, 1.92, 1.94, 1.92, 1.85, 1.91, 2.07Rheumatoid: 2.81, 4.06, 3.62, 3.27, 3.27, 3.76 Verify that 2.07 is not an outlier for the ‘normal’ group.Write the differences between physisorption and chemisorption with respect to the following: (i) Specificity (ii) Temperature dependence (iii) Reversibility and (iv) Enthalpy changeIn the preparation of a standard stock solution for protein analysis, 50 mg of bovine serum albumin (BSA) was dissolved in deionized distilled water to a final volume of 10 mL. What is the weight of BSA in 0.60 mL stock solution?
- Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.211 and A650=0.086, taking into account the dilution factor as per question 6 (100ul), and the total volume of extract as per question 4 (140ml) . Note your answer to 2 decimal placesHow can false low results due to the Hook Effect be accounted for in immunoassay testing?Calculate the Ksp for uraninite (reaction 1) and rutherfordine (reaction 2). UO2 + 4 H+ ---U4+ + 2 H2O (reaction 1) UO2CO3 ---UO22+ + CO32- (reaction 2)
- Protein ABC is a heme-containing protein responsible in electron transfer in microalgae. The target protein was extracted using ammonium sulfate fractional precipitation and quantified using Bradford assay. A set of solutions with increasing concentration was prepared using 15.00 mg/mL analogous protein standard [refer to succeeding table]. Each solution was treated with 80 μL of Bradford reagent and diluted to 1.500mL. The absorbance at 595 nm of each solution was obtained and presented below. Volume of protein std, μL A595 0 0.0002 10 0.0007 25 0.0075 50 0.0165 100 0.0356 250 0.0930 500 0.1950 750 0.3010 1000 0.4175 Report the equation of the calibration curve and proof of linearity (R2 value).I did not understand soultion for the question.. The relationship between Affinity and Association & Dissociation Constant. Four proteins (A-D) all bind the same ligand (X), with different affinities. For protein A & B we know that they have a binding site for X with a Kd (dissociation constant) of 10⁻⁵and 10⁻⁸ M, respectively. For protein C and D we know that they have a binding site for ligand X with a Ka (association constant) of 10³and 10⁵M, respectively. Which protein has the highest affinity for ligand ? Explain your reasoning. How do you make them all in same constant so that values can be compared and the one with highest Ka or lowest Kd (Highest affinity in both cases) can be ruled out.Electrophoresis of a mixture of lysine, histidine, and cysteine is carried out at pH 7.64. Describe the behavior of each amino acid under these conditions.
