What are the different technical considerations in performing the serum protein electrophoresis method? What should be done to obtain acceptable performance?.

Q: In HPLC, why is purging being done before analysis of samples. Outline the procedure on how purging…

A: High-performance liquid chromatography (HPLC) is one of the separation technique used to analyze of…

Q: How can controlled potential coulometry detect the analyte?

A: Controlled potential coulometry, as the name suggests, is a coulometry method that measures the…

Q: What is 2D gel electrophoresis

A: 2D gel electrophoresis is a technique for detection and separation of proteins. It is a combination…

Q: What would happen if we use longer columns and select adsorbing materials with 200-400 mesh sizes…

A: Solution is given in the following steps:

Q: Briefly explain how anodic stripping analysis is able to be used for quantifying DNA

A: Cathodic or anodic stripping voltammetry have been used for a highly sensitive determination of…

Q: Are the number of spots shown on SDS-PAGE electrophoresis dependant on whether the protein is a…

A: It is to explain that whether the number of spots shown on SDS-PAGE electrophoresis is dependent on…

Q: Are the analyte molecules charged or not when isoelectric focusing separation is completed? Explain…

A: The analyte particles are charged. They just go to  oppositely charged electrodes. Electrophoresis…

Q: solvent front origin

A: In thin layer chromatography the compounds separate based on polarity, the more polar compound move…

Q: Capillary electrophoresis differs from other chromatographic methods because:

A: It uses unique detectors.

Q: Thomas obtained a chromatogram for hydrocarbon analysis as below. He anticipated that his setting…

A: The main problem with this spectrum is that the peaks of the components are two closely located and…

Q: Suggest and discuss the chromatographic method for analysis of residual toxins and elevation content…

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Q: In your own words, discuss the reason why stray light should be minimized. How does stray light…

A: Stray light is defined as the light whose wavelengths lies outside the monochromator range. Stray…

Q: Briefly discuss flame ionization detectors in gas chromatography under the following headings: a)…

A: Flame ionization detectors are most sensitive gas chromatographic detectors. It measures analytes in…

Q: Adenine and caffeine have retention times of 15.50 and 17.63 min, respectively, on a 30.0-cm column.…

A:

Q: In chromatography, if the temperature of oven increases, what will be its effect on retention time…

A: Chromatography It is the separation and purification technique used in which a stationary phase and…

Q: Can be applied for the separation of ionic (nonprotein) analytes

A: Ans: IEX

Q: Explain how solid-phase microextraction works. Why is cold trapping necessary during injection with…

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Q: Consider the following setup of a capillary electrophoresis (CE) system: What are the two major…

A: Capillary electrophoresis is an analytical technique that separates ions due to their…

Q: How analytes are recovered from SFC and SFE

A: SFE is supercritical fluid extraction, in which components in a mixture are separated from each…

Q: What further testing should be done to extract and compare the composition of food dyes used In two…

A: Food coloring or the additives refers to a dye or a pigment or any substance which  imparts color to…

Q: The plot of retention time versus detector signal in chromatography called?

A: In the GC-plot, the detector response signal is at the y-axis, and the retention time is at the…

Q: Biuret Assay accurately quantify protein concentration within the range of 5-150 mg/mL. JUSTIFY THE…

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Q: Analysis of protein purity using two-dimensional electrophoresis could involve

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Q: ttering technique. If the formulation of the subject sample has included surfactants or salt…

A: The size of small particles in solution or in suspension is determine by the light scattering…

Q: Describe how you would prepare a sample for TGA analysis if it were provided in the form of: (i)…

A: Thermogravimetric analysis (TGA) of polymers is conducted to measure weight changes as a function of…

Q: The standard curve of a biuret assay gave the equation: y=0.4193x. The absorbance for Unknown A =…

A: Biuret Protein Assay is a colorimetric technique specific to proteins and peptides. Biuret is a…

Q: how might chromatography be used to analyze the composition of the dye in a marker?

A: Chromatography is a method for analyzing mixtures by separating them into the chemicals from which…

Q: What are the chief differences between the Ninhydrin and Bradford assays for amino acid…

A: Ninhydrin and Bradford assay both are performed to determine amino acids. Nin hydrin reacts with…

Q: Why should the syringe be removed from the sensor when setting the initial volume for each trial?

A: Option C

Q: What information is displayed in a reconstructed total ion chromatogram, selected ion monitoring,…

A:

Q: Derivatiztion of sample is carried out to: a) Improve analyte peak shape analyte resolution b)…

A: Derivatization of sample is carried out to, (a) Improve analyte peak shape (b) Increase the detector…

Q: Why does a molecularly imprinted polymer selectively bind a desired analyte?

A: Molecularly imprinted polymer selectively bind a desired analyte has to be explained.

Q: Can you write a report on Protein Electrophoresis, |Q&A please Aim of Experiment: Determination of…

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Q: In HPLC, why is purging or initialization being done before analysis of samples, explain.

A: ✓basically following two reasons for using purging and initialization during HPLC sample preparation…

Q: The following data give the recovery of bromide from spiked samples of vegetable matter, measured…

A:

Q: the efficiency of a chromatographic column improves the measure that a.increases the height of the…

A: To determine what improves the efficiency of a chromatographic column:

Q: For qualitative analysis with gas chromatography, the retention time of a standard is compared with…

A: Hello. Since your question has multiple parts, we will solve first question for you. If you want…

Q: by

A: Chromatography is basically that technique which allows the separation, identification, and…

Q: For what type of separation would you use ion exchange chromatography (IC)?

A: Ion exchange chromatography separates ions and polar molecules based on their affinity to the ion…

Q: Based on the given preparation procedure, identify THREE mistakes that were made.

A: High-performance liquid chromatography (HPLC) is a more enhanced chromatography with a large…

Q: Determine the order of inhibition time of hexane, hexanol and benzene compounds in the reverse phase…

A: Given compounds: Hexane, hexanol and Benzene. To determine: The order of Inhibition time in Reverse…

Q: An ion chromatograph is used for the determination of chloride ions. Seven replicate asurements of…

A: Given: Number of replicates  = 7 The replicate measurements obtained are 20, 21, 18, 22, 20, 22, and…

Q: What kind of chromatography would you for purification of full length antibody ?

A: Chromatography is used to seperate and purify mixture of species . Various type of chromatrographic…

Q: How can the mass transfer "C" term be reduced? a. increase the flow rate b. reduce the size of the…

A: In chromatography, The resistance presented during the mass transfer in between the mobile and…

Q: Two group of students wish to analyse caffeine content in tea samples. Group 1 performed the…

A: Chromatography is the laboratory technique for the separation and isolation of a variety of samples…

Q: Which of the following is TRUE regarding instrumental methods of analysis?   A. these methods have…

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Q: Name types of gels (sieving matrix) that may be used for gel filtration chromatography and discuss…

A: The types of gels for gel filtration chromatography include, Dextran Polyacrylamide beads/ agarose…

Q: FT-IR technique can be utilized for the analysis of unknown analytes by matching it with__________.…

A: