ChemistryHi, i just need some help on how to calculate the concentration of the samples? Thank you Table 2: Nelson Glucose Assay – volumes and sequence for addition of reagentsas well as glucose concentrations and absorbances for standards andsample tubesDeprot.samplesGlucose StandardsAВDE#1#2Standard/samples ()0.20.22 Nelson's copper (mL)0.50.53 Arsenomolybdate mL)0.50.54 Distilled water (mL)0.70.7Total volume (mL)1.91.9Glucose conc. (µM)75150225300Absorbance (600 nm)0.06540.2119 0.3097 0.4397 0.247610 0.0722

The question involves the beer lambert law equation: A=ε c lA is the absorbance; c= concentrationl =…

ample tub Deprot. samples Glucose Standards A B E #1 #2 O Standard/samples (r) 0.2 0.2 Nelson'scopper(mL) 0.5 0.5 3 Arsenomolybdate (mL) 0.5 0.5 4 Distilled water (mL) 0.7 0.7 Total volume (mL) 1.9 1.9 Glucose conc. (µM) 75 150 225 300 Absorbance (600 nm) 0.0654 0.2119 | 0.3097 0.4397 0.247610 0.0722

ample tub Deprot. samples Glucose Standards A B E #1 #2 O Standard/samples (r) 0.2 0.2 Nelson'scopper(mL) 0.5 0.5 3 Arsenomolybdate (mL) 0.5 0.5 4 Distilled water (mL) 0.7 0.7 Total volume (mL) 1.9 1.9 Glucose conc. (µM) 75 150 225 300 Absorbance (600 nm) 0.0654 0.2119 | 0.3097 0.4397 0.247610 0.0722

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Chemistry

Hi, i just need some help on how to calculate the concentration of the samples? Thank you

Table 2: Nelson Glucose Assay – volumes and sequence for addition of reagents
as well as glucose concentrations and absorbances for standards and
sample tubes
Deprot.
samples
Glucose Standards
A
В
D
E
#1
#2
Standard/samples ()
0.2
0.2
2 Nelson's copper (mL)
0.5
0.5
3 Arsenomolybdate mL)
0.5
0.5
4 Distilled water (mL)
0.7
0.7
Total volume (mL)
1.9
1.9
Glucose conc. (µM)
75
150
225
300
Absorbance (600 nm)
0.0654
0.2119 0.3097 0.4397 0.247610 0.0722

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