Topic: Isolation of E. coli bacteriophage   What would happen if: - the enrichment method in the isolation of bacteriophage was omitted? - the chloroform was not added to the enrichment? - the 0.1 ml lysate-E. coli mix was plated directly on top of the bottom agar?

Q: Give advantages and disadvantages of using commercially available kits for plasmid extraction over…

A: As molecular biology techniques get more sensitive, a DNA extraction kit that yields high-quality…

Q: fluorogenic methods that can be used in detecting coliforms and E.coli

A:

Q: What method is used to transfer plasmid DNA into bacteria? Group of answer choices heat shock…

A: Plasmid  It is an extra chromosomal piece of DNA found is many of the prokaryotes.

Q: Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen…

A: Rapid freezing of cells can cause ice crystals large crystals can be formed in which cells are…

Q: Five different strains of E. coli (1, 2, 3, 4 and 5) were streaked onto a series of tester plates.…

A: Biochemical pathway will be as-  MM- MM+C - MM+A - MM+B - CM Hint- the more the positive signs of…

Q: Why is the annealing temperature different between samples? How is this computed?

A: Introduction Polymerase chain reaction or PCR  laboratory technique by which millions to billions of…

Q: How to do cellulolytic characterization using a 96-well plate? What is the protocol or procedure to…

A: Cellulolytic action can be measured by an assortment of techniques that have been summed up in…

Q: Briefly explain Current Infection Marker (CIM) test, Campylobacter-Like Organism (CLO) test, and the…

A:

Q: Discuss about the reagents used for agarose gel electrophoresis

A: Gel electrophoresis is used to separate nucleic acid.

Q: 1. Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo…

A: Introduction In Virology, A Plaque-forming Unit (PFU) Is A Metric That Describes How Many Virus…

Q: Currently I am working with qPCR quantification specific for Lactobacillus bacteria. I found in some…

A: The melting curves are generated when the DNA is heated along with the fluorescent DNA-binding dye.…

Q: Transcribed Image Text:Complete the following tasks. You discovered that a species of bacteria can…

A: Introduction  Gel electrophoresis is a laboratory technique for separating DNA, RNA, and protein…

Q: Since the development of the calcium chloride transformation procedure, other procedures have been…

A: Bacterial transformation is the transfer of  DNA released from a donor bacterium into the…

Q: Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) 1. Why do you destain the gel?

A: There are various electrophoresis techniques used for the separation and identification of proteins.…

Q: Five different strains of E. coli (1, 2, 3, 4 and 5) were streaked onto a series of tester plates.…

A: Auxotrophic microorganisms cannot produce a certain organic chemical essential for their growth. The…

Q: List 2 controlled variables in the leaf disk assay. List 2 manipulated variables in the leaf disk…

A: Leaf disk assay is an experiment to determine the photosynthesis rate and oxygen production in each…

Q: Discuss the quality criteria for nucleic acid isolates. Explain the use of UV spectrometry in…

A: Nucleic acids are the macromolecules;which play important role in storing genetic information.DNA…

Q: in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this…

A: Answer: Introduction: Ribosomes are big complexes of RNA and protein which do the vital task of…

Q: Chromobacterium violaceum grows as dark purple/black colonies. Escherichia coli grows as beige/white…

A: Escherichia coli is a Gram-negative bacteria that are rod in shape. Most types of these bacteria are…

Q: Differentiate the Guanidium-thiocyanate phenol-chloroform method from the silica membrane based…

A: Nucleic acids are involved in various processes in the cell, their main role is the expression of…

Q: Which of the following is used extensively in biochemical and genetic work ? (a) Agaricus (b)…

A: All the organisms given in the question are different types of fungi. Organisms belonging to the…

Q: theraphy, What are the drawbacks of this technology? Enumerate at least 2and briefly explain ypur…

A: Gene therapy can be defined as the introduction of a new DNA into an old DNA to bring about genetic…

Q: hypothetical

A: Isolation and characterization of plant and human pathogenic bacteria from green pepper (Capsicum…

Q: What do the following abbreviations mean: DdDp, RdDp, DdRp, RdRp. Which could be provided by cells?

A: Answer: Introduction: RNA-dependent RNA polymerase (RdRp) is also called as RNA replicase, it is a…

Q: Biochemical and differential tests can be incorporated into growth media or performed on isolated…

A: It is required to provide three examples of differential test that is incorporated into growth media…

Q: COMPUTATIONAL TRUE OR FALSE Milk sample A contained an average of 53 cells per FOV at a dilution…

A: Given: Average cells, Sample A = 53 cells per FOV Sample B = 99 cells per FOV In order to get…

Q: What is the importance of performing the Modified Allen Test? Give at least three.

A: Modified Allen test: This test is done to assess the integrity of the ulnar artery, To perform this…

Q: briefly explain the principle behind this technique; gel-filtration chromatography

A: First of all, let's discuss gel-filtration chromatography. This technique is also known as the size…

Q: 100ml of LB media with 25 μg/ml of Amp and 100 μg/ml of Kan final concentration. You have 100ml of…

A: An artificial nutrient medium contains nutritional components like sugars, salts, amino acids, etc.…

Q: isolation and purification of bacteriophages from sewage through enrichment centrifugation and the…

A: Isolation of Bacteriophages Centrifuge the Sewage samples at 12000 x g for 30 minutes. It will…

Q: Five different strains of E. coli (1, 2, 3, 4 and 5) were streaked onto a series of tester plates.…

A: Having a minimal medium decides what exactly is used by the bacteria that is under study for its…

Q: colored. Interpretation: () Lactose Observed that the addition of the Benedict reagent to the…

A: Carbohydrates or carbs are macronutrients consisting of Carbon, hydrogen, and oxygen atoms. In…

Q: What are the benefits and disadvantages of IMAC for purifying proteins?

A: The purification of the proteins is performed by the use of different chromatographic procedures…

Q: Besides agarosc gel electrophoresis, there is also macromolccules. Differentiate both these methods.…

A: Agarose gel electrophoresis and SDS-PAGE are techniques for separation of macromolecules.

Q: A 10-2 is performed on a culture of bacteria in order to perform viable plate counts. From the…

A: CFU means colony forming unit. It is a measure of viable bacterial or fungal cells. In case of…

Q: How can single-cell genomics be used to addressmicrobial dark matter?

A: Microbes have a key role in plant growth, health and they also control certain climates and…

Q: Which of the following is a reason to run simultaneous tests on known positive and negative controls…

A: A negative control is a control group in an experiment that uses a treatment that  will not produce…

Q: Bradford Assay: Coomassie Blue G-250, phosphoric acid: A. Biuret Protein Assay: sodium potassium…

A: Bradford assay is a test used to quantify proteins in a sample. It uses an acidified solution of…

Q: Give a brief and clear answer, please Explain briefly the working principle and the information…

A: Spectroscopic characterization techniques The principle of spectroscopic techniques is that the…

Q: Why is the DNA polymerase used in PCR derived from an extreme thermophile bacteria species rather…

A: Answer

Q: During the Ames test, his− bacteria are exposed to a mixture of chemical compounds extracted from…

A: The Ames test, named for its developer, Bruce Ames, is a method to test chemicals for their…

Q: Process of Dpph free radical scavenging assay

A: DPPH free radical scavenging assay is a fast, basic, reasonable and broadly utilized technique to…

Q: What is the purpose of the Ames test? How are his− bacteria used in this test?

A: Ames test is also known as Bacterial reverse mutation test. It is a method to test a chemical…

Q: A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts.…

A: When the culture medium is dispersed on the viable plate, a very thin layer is formed in which…

Q: Which of the following methods for determining protein concentration is most commonly used? O Lowry…

A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: What are some similarities and differences in Bradford assay and Nanodrop (to obtain direct…

A: Protein quantification assays and their benefits and setback.

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.