Biochemical and differential tests can be incorporated into growth media or performed on isolated colonies after they have been cultured. Give three examples of a differential test that is incorporated into media and three examples that are applied after the colonies have been cultured.

Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…

A: Fluorescence quenching is a significant method for estimating restricting partiality among ligands…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: Cause-and-Effect Analysis: Given the conditions below, describe the effect on the staining procedure…

A: The differential staining seen with the Gram stain occurs because gram-positive and gram-negative…

Q: Gel used in electrophoresis is/are: a. Agarose gel b. Polyacrylamide plain gel c. Polyacrylamide SDS…

A: Electrophoresis is a method used to separate mixtures of RNA , DNA or proteins according to their…

Q: FNA

A: The above test is called the COAGULASE test. The coagulase test is a experiment to determine whether…

Q: Discuss about the reagents used for agarose gel electrophoresis

A: Gel electrophoresis is used to separate nucleic acid.

Q: List 3 advantages and 3 disadvantages of the protein assays Biuret, Folin (Lowry), and Bradford.

A: Protein assays used in determining the protein concentrations. Different protein assay techniques…

Q: Which of the following cannot be grown in artificial media and must be grown in live animals or…

A: Artificial media is a medium that is defined. This medium is chemically defined. It is a synthetic…

Q: What are the differences among native gel electrophoresis, SDS-PAGE, isoelectric focusing,…

A: The separation techniques are essential to identify and isolate biomolecules for further biological…

Q: How many microliters of the 500mM colchicine is required for making 1.5mL cell culture with 2mM…

A: Volume of cell culture = 1.5 ml = 1500 microL(V2) (1 ml = 1000 microL) Desired colchicine…

Q: Since the development of the calcium chloride transformation procedure, other procedures have been…

A: Bacterial transformation is the transfer of  DNA released from a donor bacterium into the…

Q: What is the color result of 1% solution of casein, Albumin, Gelatin in ninhydrin test? Include…

A: Ninhydrin test: Chemical test to detect presence of ammonia, primary/secondary amines, or amino…

Q: List three coating techniques used to make sol-gel coatings, and briefly describe each technique.

A: Sol-gel  Is a method of creating solid materials from tiny molecules.  The process involves the…

Q: Please explain the theoretical background (positive color reactions and what is being detected)…

A: Proteins are the polymers of amino acids. The amino acids are joined together by the peptide bonds.…

Q: Following electrophoresis of the two pGlo mini-preparation samples (both digested and undigested),…

A: Electrophoresis is a technique wherein a sample of nucleic acids or proteins can be separated on the…

Q: Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. What to do?…

A: Bradford assay is the process of Spectroscopic determination of concentration of total protein in a…

Q: Explain the test to perform by gel electrophoresis?

A: DNA is separated according to its size by a process known as Gel electrophoresis. Since DNA is a…

Q: list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet…

A: Endospores are structures produced within certain bacterial cells that allow them to survive harsh…

Q: COMPUTATIONAL TRUE OR FALSE Milk sample A contained an average of 53 cells per FOV at a dilution…

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Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…

A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…

Q: When using the blue/ white colony screening method, what do blue colonies represent ?

A: Blue- white screening is used for the identification of recombinant bacteria.

Q: What is the importance of performing the Modified Allen Test? Give at least three.

A: Modified Allen test: This test is done to assess the integrity of the ulnar artery, To perform this…

Q: If a technician examined a Kirby-Bauer assay and found individualcolonies of the plated microbe…

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: Prepare the following LB media with antibiotic added. A 100ml of LB media with 25 micro/ml of Amp…

A: Luria-Bertani (LB) broth is a liquid media comprised of several carbon and nitrogen sources. It is…

Q: briefly explain the principle behind this technique; gel-filtration chromatography

A: First of all, let's discuss gel-filtration chromatography. This technique is also known as the size…

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A: Emulsions are heterogeneous biphasic systems. Thus, there are two immiscible phases. One of the…

Q: Voges Proskauer test Does color of the medium change to red after both reagents were added and…

A: Biochemical method of bacterial identification is a classical method that is used for the…

Q: Five different strains of E. coli (1, 2, 3, 4 and 5) were streaked onto a series of tester plates.…

A: Having a minimal medium decides what exactly is used by the bacteria that is under study for its…

Q: Name the substance used as a medium in gel electrophoresis.

A: Electrophoresis is the process of separation of charged macromolecules under the influence of…

Q: Why differential centrifugation can cause contamination in pellet?

A: Differential centrifugation is a typical process used in microbiology and cytology to separate…

Q: Besides agarosc gel electrophoresis, there is also macromolccules. Differentiate both these methods.…

A: Agarose gel electrophoresis and SDS-PAGE are techniques for separation of macromolecules.

Q: WHY IN INDOLE TEST A COLORED RING FORM AT THE TOP OF THE CULTURE MEDIA BUT NOT THE WHOLE CULTURE…

A: Indole test is a biochemical test for distinguishing between Enterobacter aerogenes and Escherichia…

Q: Outline in detail the procedure for celloidin embedding .

A: Embedding is that the method during which the tissues or the specimens are capsulate in a very mass…

Q: What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail…

A: Errors and limitations of gel electrophoresis.

Q: What could be possible errors when you incorrectly identify an Unknown organism when using IMViC…

A: IMViC tests are used to identify bacteria from specimens collected from patients in majority of…

Q: What are the advantages and disadvantages of using the Slide Culture technique?

A: Slide culture technique is mainly utilized for the preparation of fugal colonies. Fungi are…

Q: Define the following terms; Sterilization, and Aseptic Technique. What are the differences between…

A: Microbes invading and proliferating within an organism's body causes infection. Microbes include…

Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…

A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…

Q: Discuss the functions of the following components in performing gel electrophoresis;…

A: Introduction: The technique of gel electrophoresis is used to separate DNA fragments based on their…

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A: Dilutions are used in microbiology to reduce bacterial concentrations to a needed concentration for…

Q: Which of the following methods for determining protein concentration is most commonly used? O Lowry…

A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Outline the techniques of two-dimensional gel electrophoresisand tandem mass spectroscopy, and…

A: Two-dimensional gel electrophoresis is the primary technique for proteomics work. It separates the…

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A: The hemocytometer is a counting chamber or slide used for the purpose of cell counting. It was…

Q: What is meant by the word sterile? Why is aseptic techniquenecessary for successful maintenance of…

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Q: The chemical precipitation method can be used for ceramic nanoparticles production. * TRUE FALSE

A: “Since you have asked multiple questions, we will solve the first question for you. If you  want any…

Q: Why rapid tests are not the gold standard in Covid19 detection. Explain.

A: Diagnosis of the Sar-Cov-2 virus is usually done by means of isolating samples collected from the…

Q: Short wavelength irradiation (~260 nm) of cells will cause which abnormality? (best answer)

A: Ultraviolet light is the part of the electromagnetic spectrum between 200 and 400 nm, with shorter…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.