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Name: Using nucleotide letters, show the kind of cut that could be made on a
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Description: Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid.What are restriction length polymorphisms, and how are they used?

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Biochemistry

Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid.What are restriction length polymorphisms, and how are they used?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 11QP: Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA...

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Similar questions

Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?
What is a restriction enzyme? How can restriction enzymes be used to splice a piece of human DNA into a plasmid?
a. Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid. b. What are restriction length polymorphisms, and how are they used?
When making recombinant DNA, why must you use the same restriction enzyme to cut the gene of interest and the plasmid?
What would happen if the restriction enzymes do not cut the DNA at specific recognition sequences?
What is a restriction digest? What does it mean if you were given a precut DNA?
In making recombinant DNA, what is the benefit of using a restriction enzyme that cuts DNA in a staggered fashion?
What is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic acids at specific sites b. to join nucleotides during transcription c. to add new nucleotides to the growing strand of DNA d. to repair breaks in sugar - phosphate backbones
In the formation of recombinant DNA, a restriction endonuclease cuts a bacterial plasmid to give sticky ends. The DNA segments that are to be added to the plasmid are cleaved with the same restriction endonuclease. What aresticky ends and why is it important that the target DNA and the plasmid it will be incorporated into have complementary sticky ends?
If you were doing a cloning experiment where a plasmid was treated with PstI but the insert DNA did not have a PstI restriction site, what restriction enzyme(s) would be suitable to treat the DNA to be inserted?
In relation to the use of restriction enzymes in recombinant DNA technology, answer the following: You have accidentally torn the labels off two tubes (tube A and tube B), each containing a different plasmid, now you do not know which plasmid is in which tube. Fortunately, you have restriction maps for both plasmids, shown in Figure below. You have the opportunity to test just one sample from one of your tubes. By utilizing agarose gel electrophoresis technique, which restriction enzyme OR combination of restriction enzymes would you use in this experiment to determine which plasmid is found in which tube?. (Hint: if you use Hind III restriction enzyme you are going to get ONE single fragment with a molecular size of → 0.5+0.3+0.2+0.4+1+1 = 3.4 kb).
Do restriction enzymes always cut the DNA at the recognition sequence?