velocity (rate of formation Velocity with 10 mg/ml ibuprofen (mM/min) [arachidonic acid] (mM) 1/[S] 1/v 1/v (mM) (mM/min with ibuprofen (mM/min of PGG,) (mM/min) 23.5 0.5 1.0 1.5 2.5 3.5 16.67 25.25 30.49 37.04 38.91 2.00 0.0426 0.0311 0.0271 0.0239 0.0227 0,0600 0.0396 1.00 0.667 0.400 0.286 32.2 36.9 41.8 44.0 0.0328 0.0270 0.0257 Use a Lineweaver-Burk plot (double reciprocal plot) on the graph paper below to determine K, and V in the absence and in the presence of ibuprofen. Show your work/reasoning, and state units for all numerical answers.
velocity (rate of formation Velocity with 10 mg/ml ibuprofen (mM/min) [arachidonic acid] (mM) 1/[S] 1/v 1/v (mM) (mM/min with ibuprofen (mM/min of PGG,) (mM/min) 23.5 0.5 1.0 1.5 2.5 3.5 16.67 25.25 30.49 37.04 38.91 2.00 0.0426 0.0311 0.0271 0.0239 0.0227 0,0600 0.0396 1.00 0.667 0.400 0.286 32.2 36.9 41.8 44.0 0.0328 0.0270 0.0257 Use a Lineweaver-Burk plot (double reciprocal plot) on the graph paper below to determine K, and V in the absence and in the presence of ibuprofen. Show your work/reasoning, and state units for all numerical answers.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
How does one plot the lineweaver burk plot using this data.
![(This is taken mainly from Lehninger Principles of Biochemistry, 6th ed., ch. 6, #12, p. 239.)
The kinetic data below are for the cyclooxygenase reaction catalyzed by prostaglandin H2 synthase
("COX"), producing prostaglandin G2, the precursor of other prostaglandins and of thromboxanes.
Velocity was measured as a function of the concentration of the substrate arachidonic acid in the
absence and in the presence of 10 mg/ml ibuprofen (Cı13H1802). The molecular mass of the
undissociated acid form of ibuprofen is 206 g/mol. (Structure of the anionic form is on p. 846 of
Lehninger Principles of Biochemistry, 6th ed.) The reciprocals of velocity and of [substrate] have
been calculated for you!
[arachidonic acid]
(mM)
1/[S]
velocity (rate
of formation
1/v
Velocity with
10 mg/ml
ibuprofen
(mM/min)
1/v
(mM):
(mM/min
with ibuprofen
of PGG.)
(mM/min
(mM/min)
23.5
32.2
36.9
0.5
2.00
0.0426
16.67
0.0600
1.0
1.00
0.0311
25.25
0.0396
1.5
0.667
0.0271
0.0239
0.0227
30.49
0.0328
0.0270
0.0257
2.5
0.400
41.8
37.04
3.5
0.286
44.0
38.91
Use a Lineweaver-Burk plot (double reciprocal plot) on the graph paper below to determine K, and
V in the absence and in the presence of ibuprofen. Show your work/reasoning, and state units
for all numerical answers.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0959128f-1417-4f4f-bd20-8f81c7fe5d61%2Fa88a156f-ad72-4269-ae0b-228d59794288%2F0g55xgev_processed.png&w=3840&q=75)
Transcribed Image Text:(This is taken mainly from Lehninger Principles of Biochemistry, 6th ed., ch. 6, #12, p. 239.)
The kinetic data below are for the cyclooxygenase reaction catalyzed by prostaglandin H2 synthase
("COX"), producing prostaglandin G2, the precursor of other prostaglandins and of thromboxanes.
Velocity was measured as a function of the concentration of the substrate arachidonic acid in the
absence and in the presence of 10 mg/ml ibuprofen (Cı13H1802). The molecular mass of the
undissociated acid form of ibuprofen is 206 g/mol. (Structure of the anionic form is on p. 846 of
Lehninger Principles of Biochemistry, 6th ed.) The reciprocals of velocity and of [substrate] have
been calculated for you!
[arachidonic acid]
(mM)
1/[S]
velocity (rate
of formation
1/v
Velocity with
10 mg/ml
ibuprofen
(mM/min)
1/v
(mM):
(mM/min
with ibuprofen
of PGG.)
(mM/min
(mM/min)
23.5
32.2
36.9
0.5
2.00
0.0426
16.67
0.0600
1.0
1.00
0.0311
25.25
0.0396
1.5
0.667
0.0271
0.0239
0.0227
30.49
0.0328
0.0270
0.0257
2.5
0.400
41.8
37.04
3.5
0.286
44.0
38.91
Use a Lineweaver-Burk plot (double reciprocal plot) on the graph paper below to determine K, and
V in the absence and in the presence of ibuprofen. Show your work/reasoning, and state units
for all numerical answers.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 6 steps with 1 images
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON