What is a guard column and why is it used in HPLC but not in GC systems?

Q: What is deionized water? What kind of impurities are not removed by deionization?

A: Purified water does not contain any impurity. There are various methods for purification of water…

Q: Given your choice of Flame AA, ICP-AE, XRF, EI-MS, or none of these, and keeping in mind that cost…

A: The function of Flame AA  is to detects the metal present in a sample. The function of ICP-AE is to…

Q: d) In a typical HPLC column, 150 atm of pressure is required to push the mobile phase through the…

A: In this question, we have to find out the correct answer of given problem by the help of the concept…

Q: Identify some advantages and limitations of using HPLC, and explain

A: When organic mixtures of compounds are nonvolatile, thermally not stable, and have high molecular…

Q: Is thin-layer chromatography a type of normal-phase chromatography or a type of reverse-phase…

A: Given that, Thin-layer chromatography is a type of normal-phase chromatography or a type of…

Q: Metal ions chelate polar groups of analyte molecules for longer retention time. Does this affect…

A: HPLC is based on the principle where the sample (analyte) is distributed between a mobile phase…

Q: Can an HPLC run be stopped during operation without impairing the separation

A: HPLC- High performance liquid chromatoghraphy It consists of two phase .One is stationary phase and…

Q: Which statement is true? Statement 1: Since tiny amounts of material are exposed on an open…

A: The answer of the above question is given in step 2

Q: . What is lon chromatography?

A: chromatography  : Chromatography can be defined as the chromatography is a physical technique of…

Q: Differentiate between isocratic elution and gradient elution. Why is gradient elution sometimes a…

A:

Q: .Which of the following solvents is not commonly used in reversed-phase HPLC? a) Ethanol Water b)…

A: The solvent which is not used commonly in HPLC has to be given,

Q: why is acetic acid used in the mobile phase in HPLC?

A: Acetic acid can be used as a mobile phase additive to improve the separation and resolution of the…

Q: Explain the importance of controlling mobile phase pH in reversed phase HPLC and how this is…

A: When samples contain ionisable compounds, mobile phase pH can be one of the most important variables…

Q: igh-performance liquid chromatography would never be applied in drug analysis This statement is…

A: Given : We have to tell whether the statement is true or false.

Q: What types of columns are used in HPLC in chromatography?

A: HPLC stands for high performance liquid chromatography  which is used as a separation technique in…

Q: 13. Consider the TLC plate below to answer the question. What is the Rf of the top component? Note:…

A: RF factor is given by the distance travelled by the solute divided by distance traveled by solvent .…

Q: Differentiate normal phase chromatography from reverse phase chromatography. From which of the two…

A: The major difference between normal phase chromatography and reverse phase photography is, Normal…

Q: 2. a) What is a bonded phase in HPLC? b) Give an example of a bonded phase in reversed-phase liquid…

A: Bonded Phase is the compound that is directly attached to the stationary phase in the liquid…

Q: A chromatography column would be most efficient with which of the plate heights? 0.50 mm 0.10 mm O…

A: Correct answer is option B i.e 0.10 mm

Q: What is Ultra-Performance Liquid Chromatography (UPLC)? What are the differences and advantages of…

A: The newest technology in liquid chromatography-based analysis is Ultra Performance Liquid…

Q: Explain why a chromatographic separation normally has an optimum flow rate that gives the best…

A: When an fast flow rate is maintained, the band is not allowed to spend much time within the column…

Q: How resolution in HPLC analysis can be improved?

A: HPLC i.e  high-performance liquid chromatography is an analytical method which is being used to…

Q: Differentiate between gel filtration and gel permeation chromatography.

A: Given : We have to tell what is the difference between gel filtration and gel permeation method.

Q: 3 Compare: a) Capillary Electrophoresis with GC and HPLC. b) Separation Versus Preconcentration.

A:

Q: Give 2 examples of High performance liquid chromatography (HPLC) on how or when it is recommended to…

A: High performance liquid chromatography is the modification of liquid chromatography. Now a days it…

Q: It is usually recommended to clean the glass surfaces of a refractometer with ethanol or isopropyl…

A: Refractometer is a device that measure the degree to which light changes direction called as angle…

Q: Explain the term gradient elution mode in HPLC

A: Given : We have to tell about gradient elution term .

Q: Why are the application of TGA more limited than those for DSC?

A: TGA is one of the thermogravimetric techniques. In this process changes in chemical properties and…

Q: 1. You know that these types of compounds can be analyzed by either GC or HPLC but you are told by…

A: GC can only apply to  analyse volatile substance while HPLC can handle any soluble compound…

Q: 1- What are the main two types of columns in the HPLC? 2-What are the most disadvantages of…

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: 11. (8pts) how can silica gel Fsa TLC plates can be visualized ( give two methods? :

A: In the given question we have to visualise the F254 TLC plate, there are two method by which we can…

Q: Write down three differences between GC and HPLC (GC = gas chromatography) (HPLC =…

A: 1. High Performance Liquid Chromatography uses a liquid mobile phase and gas chromatography uses a…

Q: Define the following terms used in HPLC:                      (d) sampling loops             (e)…

A: The following terms used in HPLC can be defined as :

Q: Why are some of the detectors listed unsuitable for HPLC? Which of the GC detectors ) in the table…

A: The function of detectors used in high-performance liquid chromatography is to monitor the mobile…

Q: describe different type of degasser in HPLC system, and why degassing is important in the middle of…

A: Degasser is  very useful in HPLC .the main use of degasser in HPLC are  hellium sparging, sonication…

Q: The total retention time of dodecane on a 15 m x 0.25 mm i.d. DB-5 GC column was 8.0 min. The column…

A: Given: Number of theoretical plates=N=25600Retention time=tR=8.0 min

Q: Choose the appropriate techniques for degassing the mobile phase of HPLC. I. Ultrasonic II. Boiling…

A:

Q: Paper Chromatography and TLC are examples of planar chromatography. True or FalsE?

A:

Q: What is Jon chromatographys

A: Given To know about the chromatography

Q: I) Choose the Correct Answer from the Multiple-Choice List (10) 1. Which of the following is not a…

A: Hello. Since you have posted multiple questions and not specified which question needs to be solved,…

Q: Compare gradient and isocratic mode in HPLC.

A: Gradient and isocratic mode in HPLC are the two different modes of operating condition in HPLC.

Q: Explain why it is needed to filter the solvent before introducing it to the HPLC system

A: Basically there are following two reasons for using filtration during HPLC sample preparation are…

Q: . Compare HPLC, HPTLC and GC chromatography

A: Chromatography is a physical separation method that is used to separate and identify the components…

Q: In TLC, point of origin refers to O The bhottom edge of the TLC plnto.

A: Question 1: Option 1 is the correct answer. The point of origin was sketched very lightly with…

Q: Gel permeation chromatography depends on the degree of species segregation of a sample

A: Given         Gel permeation chromatography depends on the degree of species segregation of a sample…

Q: Why are peak areas used instead of peak height in Reverse Phase HPLC of Caffeine and Benzoic Acid?

A: In case of carrying out HPCL of caffeine and benzoic acid, peak heights are better option for…