Macroscale and Microscale Organic Experiments
7th Edition
ISBN: 9781305577190
Author: Kenneth L. Williamson, Katherine M. Masters
Publisher: Brooks Cole
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Textbook Question
Chapter 8, Problem 7Q
What will be the result of applying too much compound to a TLC plate?
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What will be the appearance of a TLC plate if a solvent of too low polarity is used for the development? too high polarity?
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What happens if the spots are made too large when preparing a TLC plate for development?
Chapter 8 Solutions
Macroscale and Microscale Organic Experiments
Ch. 8 - Prob. 1QCh. 8 - Prob. 2QCh. 8 - What problem will ensue if the level of the...Ch. 8 - Prob. 4QCh. 8 - Prob. 5QCh. 8 - Arrange the following in order of increasing Rf...Ch. 8 - What will be the result of applying too much...Ch. 8 - Prob. 8QCh. 8 - Prob. 9QCh. 8 - A TLC plate showed two spots with Rf values of...
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- Why is it important to handle the TLC plate in such a way that your fingers do not come in contact with the coated surface of the plate?arrow_forwardThe solvent level in the development chamber should be above the level of the spotted compounds on the TLC plate. Group of answer choices True Falsearrow_forwardBefore developing a TLC plate you should _______.a. Make sure to mark the origin with a pen b. Make sure to mark the origin with a pencil c. Make sure the filter is all the way around the inside of the developing chamberarrow_forward
- 1. Which property of compounds is responsible for the separation in a TLC? Explain with an example.2. How does the separation occur during a TLC? (Hints: TLC plate and silica layer)arrow_forwardWhat is the purpose of evaporating off a majority of the pentane solution before spotting the TLC plate? What would be the consequence of skipping the evaporation step?arrow_forwardWhy are some of the detectors listed unsuitable for HPLC? Which of the GC detectors ) in the table below ) are suitable for HPLC?arrow_forward
- What is a guard column and why is it used in HPLC but not in GC systems?arrow_forwardDemonstrate how to make TLC, and analyze the sample for any purpose, which deemed suitable for youarrow_forward1. TLC a) What are the results of loading too much compound to a TLC plate?b) What would happen if you added too little compound to a TLC Plate?arrow_forward
- When would using Thin Layer Chromotography (TLC) NOT be useful in conducting an oxidation reaction? What errors could happen or what drawbacks does this method have?arrow_forwardIn order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc,)arrow_forwardExplain why it is needed to filter the solvent before introducing it to the HPLC systemarrow_forward
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