What is the main factor that keeps an integrated Lambda prophage from excising and entering lytic growth? the presence of antibiotics to maintain selective pressure O high levels of bacterial cell metabolism O replication of plasmid DNA O continued production of the Cl repressor protein
Q: In what environment can viruses replicate themselves? O Inside the cells of living organisms O…
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Q: Certain environmental conditions such as exposure to UV light areknown to activate lysogenic λ…
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Q: The THIRD stage of bacteriophage lytic reproductive cycle is Cell lysis Biosynthesis O Attachment O…
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A: Transduction is that process by which the foreign DNA is inserted into the cell by virus.
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Q: describe F plasmid
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- If a single bacteriophage infects one E. coli cell present in a cultureof bacteria and, upon lysis, yields 200 viable viruses, howmany phages will exist in a single plaque if three more lytic cyclesoccur?Which of the following is true of Generalized transduction? O creates defective virions that are missing the entire phage genome phage and host DNA are both packed in the capsid by error Cha type of Hirgone transfer Frequency is I in 10¹ O occurs during the lysogenic cycleHost DNA is digested into small pieces, which are occasionally assembled with phage proteins, creating a phage with bacterial chromosomal DNA. If the breakage of the chromosomal DNA is not random (i.e., it is more likely tobreak at certain spots as opposed to other spots), how might suchnonrandom breakage affect cotransduction frequency?
- During the lytic replication cycle of the bacteriophage T4, the phage _________ remains outside the cell while the phage ________ enters the cell. A) capsid protein… tail fibers B) DNA… protein C) envelope… DNA D) enzymes… ribosome E) protein… genetic material F) RNA… capsid proteinAs described in Figure, host DNA is hydrolyzed into smallpieces, which are occasionally assembled with phage proteins, creatinga phage with bacterial chromosomal DNA. If the breakage ofthe chromosomal DNA is not random (i.e., it is more likely tobreak at certain spots as opposed to other spots), how might nonrandombreakage affect cotransduction frequency?Figure 21.10 Which of the following statements is false? In the lytic cycle, new phages are produced and released into the environment. In the lysogenic cycle, phage DNA is incorporated into the host genome. An environmental stressor can cause the phage to initiate the lysogenic cycle. Cell lysis only occurs in the lytic cycle.
- In _______, viral DNA becomes integrated into a bacterial chromosome and is passed to descendant cells. a. binary fission c. the lysogenic pathway b. the lytic pathway d. conjugationWhy is a webbed plate capable of producing a higher lysate titer (pfu/mL) than a cleared plate? a.) A webbed plate has plaques that have gone through more rounds of replication because the number of host cells did not limit phage replication, creating exponentially more phage particles b.) A webbed plate has no more host available for replication, meaning the plate is full of exponentially higher amounts of phage c.) A cleared plate has less bacteria than a webbed plate, so there are more plaques present d.) A cleared plate contains more phage than a webbed plate because it started with a more concentrated phage sampleBacteriophage T4 lacks its own RNA polymerase.How do T4 genes get expressed or converted to mRNA?What host barriers must be broken before release ofvirions from the host cell?
- Prophages and proviruses have been described as being similar to bacterial plasmids. How are prophages and proviruses similar to plasmids? How are they different from plasmids? – Review the structure and function of prophages, proviruses and plasmids, how do they replicate, where are they located within the host.Compare and contrast the mechanism of replication of T4 vs. filamentous phages.Certain environmental conditions such as exposure to UV light areknown to activate lysogenic λ prophages and cause them to progressinto the lytic cycle. UV light initially causes the repressor protein to be proteolytically degraded. Make a flow diagram showing the subsequent events that lead to the lytic cycle. (Note: The xis gene codes for an enzyme that is necessary to excise the λ prophage from the E. coli chromosome. The enzyme integrase is also necessary for this excision.)