Q: Explain the reasons you would use a negative stain?
A: In a negative stain the slide is actually stained. This is because the negatively charged stain…
Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain
A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…
Q: Why is heat necessary in spore staining?
A: BASIC INFORMATION SPORULATION It is a process during which spores are formed. We can find it in…
Q: Crystal violet is an example of what type of stain?
A: A stain is used to clearly view a specimen under the microscope. For staining, a smear of the…
Q: Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin
A: The living cells can be seen under a microscope. The cells is not visible properly as it is…
Q: hich Is The More Important Step In Gram Stain?
A: Hans Christian Gram invented the Gram stain in 1884. (Gram,1884).
Q: Why are basic dyes more effective for bacterial staining than acidic dyes?
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: Explain the function of water in spore staining.
A: Spores are microscopic units that are accountable for asexual reproduction. Several species of…
Q: For a stain to be considered differential, what must it do?
A: Differential stain uses more than two stains.
Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…
A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…
Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
A: bacteria like Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…
Q: Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria…
A: The gram-positive and negative cells differ in their cell wall composition.
Q: what are the regeants and results for Mann’s methyl blue-eosin stain
A: Mann's methyl blue eosin strain- is formulated to demonstrate Negri bodies in the tissue section it…
Q: What makes Mycobacterium resistant to staining?
A: Staining is a technique used in slide preparation to color the specimen.With light microscopy,…
Q: What is a differential stain? why is it used?
A: Differential stain is a type of stain use to identify organisms.
Q: What is the principle of Giemsa staining and components of Giemsa stain?
A: The cells are the basic structural and fundamental unit of the living system. It consists of…
Q: What is the difference between simple stains anddifferential stains?
A: Simple stains : It generally make all of the organisms in a sample appear to be the same color even…
Q: What is meant by Gram positive? Explain the mechanism of Gram positive staining.
A: Microorganisms or microbes are tangible objects that appear under a microscope. Large classes of…
Q: How are negative stains prepared?Name two applications for which this type of stain especially…
A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…
Q: why does sometimes acid-fast bacteria accept the spore stain and spores accept the acid-fast stain.
A: Acid-fast staining technique is a differential staining technique. The bacteria which get stained by…
Q: What are the functions of a primary stain and a counterstain?
A: The main stain and the counterstain must be of different colours so that the two forms of bacterial…
Q: What external bacterial cell structures can be demonstrated by a negative stain?
A: The negative stain is useful in studying the morphology of bacterial cells. It also characterize…
Q: What are the Types of Differential Stains? explain with an example.
A: Bacteria are prokaryotes that are devoid of any membrane-bound organelles. They are differentiated…
Q: What would be the result of the gram stain if acid alcohol is used as a decolorizer rather than the…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?
A: Kinyoun staining is a technique used to stain bacteria. Specifically, acid-fast species of the…
Q: Could the Gram stain be used to diagnose the fl u? Why or why not?
A: Gram staining : It is a technique used for differentiation between two large group of bacteria based…
Q: Why is it necessary to perform a negative stain to visualize capsules?
A: Introduction: Negative staining: This uses an acidic stain. The results yield a clear cell with a…
Q: What is an example of a negative stain?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: What is the accepted theory regarding the mechanism of the Gram stain?
A: Bacteria on the basis of the structural organization of their cell wall are of two types:…
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: Why does fixation of the positive dye help bacteria stick to the slides?
A: Dyes in microbiology are for staining cells; they are used based on the chemical properties and the…
Q: What is the name of bacteria for each gram staining and identify the shape and the arrangement?
A: Gram staining is the method of identification between the gram positive and gram negative bacteria.…
Q: What is the purpose of the steam in spore stain?
A: Small tiny objects which are not viewed by the naked eye are called microbes. The microbes are seen…
Q: What stain is used for mycobacterium?
A: The staining is very important process for observing an organism under microscope. The staining of…
Q: What is the main advantage of using the slide culture technique for identifying molds?
A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…
Q: why the Gram stain can differentiate between Gram positive and Gramnegative cells?
A: *Gram staining is a technique used to differentiate two groups of bacteria into gram positive and…
Q: What is the difference between Giemsa and Wright stain?
A: Staining is a technique used to enhance contrast in samples, generally at the microscopic level.…
Q: What is the secondary stain in the spore stain?
A: Endospores are the structures that are formed by bacteria during unfavorable conditions (lack of…
Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: What is a crystal violet stain and its purpose?
A: Crystal violet is that the name given to the cluster of similar organic compounds that are used as…
Q: what color are endospores at the conclusion of the endospore stain?
A: Endospores are non-reproductive structures formed by the bacteria when they experience challenging…
Q: what is the function of each one of the Gram-stain reagents?
A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…
Q: What types of compounds make up the gels used in electrophoresis?
A: The types of macromolecules usually separated on Agarose gel are : Nucleic acids-mainly DNA…
Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…
A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…
Q: Why can't gram staining be used for mycobacterium?
A: Gram staining is one of the staining techniques. This is usually used for differentiating between…
Q: what is the theory about the mechanism of the gram-stain reaction
A: Introduction :- A Gram stain test looks for bacteria in areas where an infection is thought to have…
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: What type of chromophore is associated with a negative stain?
A: Bacteria are unicellular prokaryotic organisms that maintain a definite shape since they possess the…
Q: Why do acidic dyes not stain bacterial cells?
A: Stains may be divided into two classes based on the nature of their charged group Basic/ cationic…
What is the mordant in the spore stain?
A mordant is any substance, which is used as a fixative to bind the dye on the substance.
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