What is the purpose of inverting (or siding up) an inoculated plate when we place it inside incubator?
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What is the purpose of inverting (or siding up) an inoculated plate when we place it inside incubator?
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- In aseptic technique, What will/may happen if you open a solid plate and not pass the inoculating loop or inoculating needle over a flame to take a colony or when you are about to inoculate on it?What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of an E-test strip.In aseptic technique, what will happen if the inoculated solid plated media is not inverted prior to incubation?
- during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.Why is it necessary to inoculate the KIA medium all the way down to the bottom of the tube during inoculation?Why are plates incubated in a candle jar or CO2 incubator?
- Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration.Suppose your Professor handed you a test tube with 2.0mL of E.coli broth culture and told you to make a 10-1 dilution of the ENTIRE culture. Explain how you would do this.What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?
- In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?What conclusion would you draw if the numbers of bacterial colonies in were the same on the control plate and the treatment plate? Explain your reasoning.What is the purpose of flaming an inoculating loop? How will you know when you have flamed the loop or needle long enough? Why is it necessary to cool the inoculating loop prior to obtaining the bacterial sample? In which direction should you move the inoculating loop in the Bunsen burner flame? (i.e. from the handle to the loop or from the loop to the handle)