What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution to a sterile petri dish and then added molten agar to make a pour plate? O 1) 106 O 2) 10-7 O 3) 107 O4) 4) 10-5 O 5) 10-6
Q: starting with four bacterial cells per milliliter in a rich nutrient medium, with a 1-hour lag phase…
A: Bacterial Growth --Bacterial Cell growth is proliferation of bacterium in to daughter cells ,…
Q: what might be the consequences of storaging coconut water at room temperature after collection and…
A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…
Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…
A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…
Q: Since there are still bacteria left in pasteurized milk, why is it not routinely sterilized instead…
A: Hello! Since you have posted multiple questions, we are answering only first question. If you need…
Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: What is this test? What coated the bottom of the wells? What were we testing for? How did we know it…
A: The test is ELISA. It is an Enzyme linked Immunosorbant Assay, which is used in many applications.…
Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: Why do you have to boil the agar solution before dispensing it into tubes?
A: Agar is the medium which can used to grow the micro organisms in petri plates. The selected…
Q: Can culture media be sterilized in the hot air sterilizer? Why or why not?
A: Hot air sterilization is a procedure in which the sterilization of the objects are done using hot…
Q: Why is it important to keep the culture at the correct incubation temperature when measuring the…
A: Generation time is the time which is taken by the bacteria to double in number during a specified…
Q: Can you tell IF the pasteurization process is working in this milk producing plant? How can you…
A: Pasteurization is a process in which milk or milk product is heated to a certain temperature for a…
Q: Discuss two mechanized /automated process that are used in modern broiler production?
A: Poultry farming is defined as a term for rearing and keeping of birds such as fowl, duck and hen for…
Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…
A: Making a solution thinner or less dense is the procedure of dilution. Serial dilutions (also known…
Q: Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition.…
A: A growth medium is a also known as a culture medium which can be solid, liquid, and even…
Q: Why after a certain period the growth rate of the culture decreases until growth stops?
A: Warmth, dampness, pH levels and oxygen levels are the four major physical and chemical components…
Q: What is differential medium and differential media? Explain the difference ?
A: Difference between differential medumium - .- A differential medium support the expansion of any…
Q: Why should culture media after inoculation be incubated at an optimal temperature immediately?
A: Culture media, also called as growth media, are particular combinations of nutrients and other…
Q: why is it important that a small amount of inoculum be used in preparation of an agar slant
A: Agar is a jellylike substance derived from the purified cell walls of red algae. It is added to…
Q: What would you expect to see if you cultured Gram-positive, lactose-fermenting bacteria on MacConkey…
A: Given: What would happen if you culture gram positive, lactose fermenting bacteria in MacConkey…
Q: give one reason why is it necessary to keep stirring the agar medium mixture in a beaker when…
A: Agar is a heteropolysaccharide, which is composed of different carbohydrate units. Agar is obtained…
Q: Assume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30…
A: Binary fission is the most common method of reproduction among bacteria. In this process, a single…
Q: What would your final dilution be if you transferred 0.1 mL of a 10-° dilution to a sterile petri…
A: Dilution is a process by which solution (water or diluents) are added to the solvent (main solution)…
Q: Of what practical importance are air borne microorganisms to the laboratory workers? What…
A: Questions related to the microbiology labs. Note: each questions are answered under particular…
Q: What makes agar good support for microbial growth?
A: Microbial culture is a method of multiplying microbial organisms by letting them reproduce in…
Q: WHAT IS THE TYPE OF Growth patterns on Agar Slant and Broth Medium
A: Growth and morphogenesis of plant is determined by the composition of culture media. There are few…
Q: What is the advantage of the plating method over an electronic cell counting method in counting…
A: Bacteria culturing is done in order to grow the bacterial strains that are able to show resistance…
Q: You perform a nitrate test. After adding solutions A and B, the broth remains colorless. Why should…
A: Nitrate test helps us to determine the production of nitrate reductase enzyme. By the activity of…
Q: You were instructed to add 1.0 mL out of 4.0 mL of an undiluted sample to 99 mL of sterile diluent.…
A: The dilution factor can be defined as the ratio of Volume of the initial solution to the volume of…
Q: What is the main difference between plates before and after centrifugation? What may be the purpose…
A: Answer: CENTRIFUGATION = It is the process involving mechanical force that is centrifugal force,…
Q: Why is it necessary to store the molten agar in a water bath at 50 degrees Celsius?
A: Introduction:- Agar is made up of two polysaccharides: agarose and agaropectin, with agarose…
Q: What are the culture media that can be used for cultivating non-specific and specific…
A: A microbiological culture medium is a substance that promotes the growth, support, and survival of…
Q: how could you determine if there is any contamination on your agar?
A: Agar consists of two polysaccharides, agarose and agaropectin, but agarose making up about 70% of…
Q: Why should you filter some liquids for sterilization, rather than autoclaving them?
A: To control microbial growth or contamination there are a number of aseptic techniques developed that…
Q: You have a starter culture containing 8 x 109 cells per mL, from which you take 10 mL to inoculate a…
A: The generation time of culture is considered to be the time at which it doubles and the mean growth…
Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…
A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…
Q: ar contains salt to prevent the growth of most microbes and mannitol plus a pH indicator to detect…
A: In order to produce and culture different types of microbial species, a culture media is required.…
Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. You then…
A: A serial dilution is any dilution in which the concentration is decreased by the same factor in each…
Q: How many different types of colonies can you find on the blood agar plate? describe each. (As shown…
A: Blood agar is general medium that is provided with the nutrients and can be use as a general growth…
Q: Why are plated media stored in an inverted position?
A: Plated media enables the isolation of microorganisms from samples or specimens.
Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.
A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…
Q: What is the meaning of the word sterile? What would happen if culture media freshly prepared were…
A: Sterile It refers to the state of being free from any bacteria or any other living microorganisms.…
Q: In the conduct of serial dilution, which of the following tools is NOT necessary? a. Agar…
A:
Q: Which piece of equipment sterilizes hospital tools and devices and how does it sterilize things? Are…
A: Sterilizing pieces of equipment are very important in modern healthcare settings. It is necessary…
Q: If you suspect that your microbial culture growing in nutrient broth is contaminated, what can you…
A: The contamination refers to the growth of one or more undesired cultures in your pure sample. It can…
Q: How would you inoculate a plate to get a 1:10 dilution? A 1:100 dilution?
A: Inoculation is defined as the process of introduction of microorganisms into a culture for their…
Trending now
This is a popular solution!
Step by step
Solved in 3 steps with 1 images
- What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a sterile petri dish and then added molten agar to make a pour plate?Why do you have to boil the agar solution before dispensing it into tubes?If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar plate, what is the final dilution?
- How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long should you wait for bacterial colonies to grow on agar plates? What is the appropriate temperature?.Serial dilution is recommended prior to pour and spread plate. Why is this so?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?
- In spread plate method, colonies form within the agar and agar surface. In pour plate method, previously prepared agar plates are not required. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.Noah wanted to transfer Staphylococcus aureus from a broth to an agar plate. He picked up the broth culture, removed the cap, and flamed the mouth of the tube. He inserted an inoculating loop to obtain a bacterial sample. Then, he flamed the mouth of the tube and replaced the cap. Noah opened the lid of a labeled agar plate diagonally and used the loop to streak the surface of the agar. After closing the lid, he flamed the loop in an incinerator and put it back in its container. The plate was incubated upside down for 24–48 hours. What did Noah do wrong in this transfer? a. He did not use the transfer tool correctly. b. He did not handle the culture tube correctly. c. He did not handle the agar plate correctly. d. He used the wrong tool in transfer.Discuss two mechanized /automated process that are used in modern broiler production?