Number 5 please 5. What reaction is taking place in the above activity? EFFECT OF ENΖΥΜΕ CONCENTRATIOΝ ON ΕNZΥΜΕ ACTIVITYIntroduction:Enzymes are highly specialized class of proteins. They catalyze thousands ofchemical reactions with remarkable property of speeding up chemical reactions. Theycombine with the reactants to produce a transition state having less energy than thetransition state of uncatalyzed reaction. When reaction products are formed the freecatalyst is regenerated.The rate of enzyme catalyzed reaction is determined by the concentration ofthe enzyme used. The more concentrated the enzyme used, the faster is the reaction.Objectives: At the end of the activity, the student can:1. Determine the effect of different concentration of enzyme on reactionrate2. Compute for the concentration of enzyme present in each tube.3. Determine in which concentration reaction rate is fastest and slowest.Reagents: Saliva, 6 ml 1% starch solution, 6 drops Iz in KI, 6 ml benedict's reagent.Materials: 12 Test tube (20 ml), 10 ml graduated cylinder, test tube rack, brush, 250ml beaker, tripod, wire gauze, thermometer, alcohol lampProcedure:1. Prepare 6 test tubes and place 1 ml saliva in each.2. Add 1ml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml totube 5 and 10 ml to tube 6. Mix thoroughly.3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) andadd 1 ml of 1% starch paste. Mix well and heat in a water bath withtemperature maintained at 40°C for 30 minutes.4. Divide each of the contents of each tube into 2 and test with:a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KIand note the color produced. Compare the intensity of color in each of the6 tubes.b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half ofeach tube and heat in a boiling water bath for 3 minutes. Note color ofprecipitate.5. Rank each tube according to decreasing reaction rate one (1) being the fastestto hydrolyze and 6 being the slowest.

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Enzymes are the biocatalysts that speed up biochemical reactions at optimum pH and temperature. The…