What would happen to a human red blood cell if it were placed in the following solutions? Explain your answer for each scenario. The salt (NaCl) concentration of a human red blood cell is approximately 0.9%. a. 0% NaCl b. 0.9% NaCl c. 10% NaCl
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- What would happen to a human red blood cell if it were placed in the following solutions? Explain your answer for each scenario. The salt (NaCl) concentration of a human red blood cell is approximately 0.9%.
a. 0% NaCl
b. 0.9% NaCl
c. 10% NaCl
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- Phosphate buffered saline (PBS) is a physiological buffer often used in biology experiments. Because the concentrations are very low and small amounts are difficult to weigh out, it is often prepared as a 10X stock solution meaning that it is prepared at 10 times the working concentration which is 1X. 1X PBS is made up of Na2HPO4 10 mM (MW 141.9 g.mol-1); KH2PO4 8 mM (MW 136.08 g.mol-1), NaCl 137 mM (MW 58.44 g.mol-1) and KCl 74.5 mM (MW 74.5 g.mol-1). You decide to prepare a 1 L 10X solution of PBS. What is the Molar (M) concentration of each of the components in you 10X stock. Write your answers in the table below. How much of each of the components should you weigh out to prepare 1L of the 10X stock solutions. Write your answers in the table below.A researcher prepares 100 mL of aqueous solution containing 0.1 g of a protein. The researcher then uses an experimental apparatus ,where he notes that, at room temperature (300K) and atmospheric pressure (101.325 kPa), distilled water flows naturally into the solution compartment. However, when he applies an extra pressure of 1000 Pa he observes that the flow of distilled water ceases and the system is in balance. Calculate the molar mass of this protein, in kDa (1 Da = 1 g/ mol); Why does the flow of distilled water cease with the application of the pressure of 1000 Pa? Explain in terms of potential chemicals. %3DYou just made a 1.5M permanganate solution. What concentration is your potassium permanganate solution in percent? "K: 39.10 g/mole" "Mn: 54.94 g/mole" "O: 16 g/mole" "MW of KMnO4 is 158.04 g/mole" O a. 0.24% Ob. 20.1% O c. 15.8% O d. 23.7% O e. 2%
- An aqueous solution contains 0.34 M potassium cyanide. One liter of this solution could be converted into a buffer by the addition of: (Assume that the volume remains constant as each substance is added.) 0.34 mol HCI04 0.16 mol NaOH 0.33 mol KCIO4 0.16 mol HCIO4 0.33 mol HCNYou dissolve a 3.435 gram sample of potato in acid to determine the levels of solanine (the reason why green potatoes are poisonous) and diluted it to a total volume of 500.0 mL. You have also created a standard curve as outlined below. Solve for the ug/gram concentration of solanine in the potato sample. ppm Solanine Std. Abs 0.213 5 0.315 10 0.423 15 0.532 Unknown sample 0.314 Your Answer:Consider a buffer solution that contains 0.55 M NH2CH2CO2H and 0.35 M NH2CH2CO2Na. pKa(NH2CH2CO2H)=9.88. a. Calculate its pH. b. Calculate the change in pH if 0.155 g of solid NaOH is added to 250 mL of this solution. c. If the acceptable buffer range of the solution is ±0.10 pH units, calculate how many moles of H3O+ can be neutralized by 250 mL of the initial buffer.
- A mixture of two proteins (both with mass of 50 kDa) with an molecular isoelectric points of 7.8 and 8.9, respectively, is in a buffered solution at pH 7. Which type of liquid chromatography would be used to separate these two proteins? O a. O b. O C. d. e. anion exchange chromatography size-exclusion column gel filtration Ni-NTA column cation exchange chromatographyThere are two solutions with different pH levels. Solution A is neutral, with a pH of 7. Solution B is slightly alkaline, with a pH above 7. Two beakers are filled with of each solution (one for each solution). A phosphate buffer was added to it and CO2 was blown into it until there was a change of color meaning a change in pH. (this experiment is to see how the CO2 from the lungs can cause a pH change in water) Why does it take so long for the solutions to change color? Relate your answer to the equation (H2O + CO2 ⇌ H2CO3 ⇌ HCO3- + H+ ) and to the presence of the buffer.Mammalian blood contains the equivalent of 0.15M NaCl. Seawater contains the equivalent of 0.45 M NaCl. What will happen if red blood cells are transferred to seawater? The blood cells will take up water, swell, and eventually burst. Water will leave the cells, causing them to shrivel and collapse. NaCl will be exported from the red blood cells by facilitated diffusion. NaCl will passively diffuse into the red blood cells.
- The normal range of the sodium electrolyte in the body is 134 to 145 mEq/L. The term mEq is dependent on the charge of the ion. Since sodium is a +1 ion, 134 mEq/L is the same as 134 mmol/L. An ion that has a greater positive or negative charge; however, will have 1 mEq for each positive or negative charge of the ion for every 1 mmol. For example, for Ca+2, 2mEq/1mmol. If the standard range of Magnesium in the body is 0.70 to 0.95 mmol/L, convert this value into mEq/L for the Mg2+ ion. (Use dimensional analysis to figure this out)White blod cell concentration in normal blood is approx 12,000 cells/mm^3. How many white blood cells does a normal adult with 5.0L of blood have? Express answer in scientific notation.A 5% dextrose in 1/2 normal saline (D5 1/2 NS) solution is commonly administered to patients needing post-operative IV fluids. How could you prepare 500 mL of this solution? Dextrose is 5% (m/v) and the NaCl is 0.45% (m/v).