Indicate the contrast statements of the following chromatographic techniques ofseparation.A. A separation of components mixture based on migration rates in a stationary phaseby a gaseous or liquid mobile phase.B. The supported materials coated of flat plate or in fibres of paper stationary phase isknown as planar chromatography.C. The rate of migration of molecules or analytes is depending on the relative solubilityof the solute in the stationary phase and mobile phase.D. Involve an electronic transition of valence shell electron in free atoms to provideanalytical information about sample composition.

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Answered: Indicate the contrast statements of the…

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter8: Forms Of Drugs And How They Act

Section: Chapter Questions

Problem 30RQ

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For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. 7. If the red cell suspension is the stock solution, what is being quantified in this test? What diluent/reactant should be used to detect your answer in number 7?
A 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?
Describe the effects of a lethal dose of ionizingradiation at the molecular level. What type of filterwould be used to filter sterilize a heat-sensitive liquid?
Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA    standard solution (μL) Protein content(μg/mL) Absorbance value        At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380         Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…
The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1  = activity of analyte solution   a2  = activity of internal solution How will measured pH value be affected vs “real” pH if the temperature of the sample is 30C when pH was measured ? a.measured pH is lower than real pH b.impact can not be determined c.measured pH is higher than real pH d.measured pH is same as real pH
The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1  = activity of analyte solution a2  = activity of internal solution How will measured pH value be affected vs “real” pH if HCl in pH electrode, became 0.15M instead of 0.1M ? a.impact can not be determined b.measured pH is higher than "real" pH. c.measured pH is lower than "real" pH. d.measured pH is same as "real" pH.
Describe step by step process of: a. Ion-exchange Chromatography b. Hydrophobic Interaction Chromatography c. Affinity Chromatography d. Reversed-Phase Chromatography e. Principle of Polyacrylamide Gel Electrophoresis f. Isoelectric Focusing g. Protein Sequencing
A chromatographic separation of four compounds gave the following results. The dead time (tm) is 16.7 sec. (a) What is the capacity factor for analyte 4? (b) What is the resolution factor for analytes 3 and 4?
Determine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis.
Why does eluent strength increase as solvent becomesless polar in reversed-phase chromatography, whereas eluentstrength increases as solvent becomes more polar in normal-phase chromatography? What kind of gradient is used in supercritical fluid chromatography?
The structural elucidation of cellular proteins will help to better understand cell physiology. This can be achieved by a. Subcellular fractionation & ion exchange chromatography b. Polyacrylamide gel electrophoresis & nuclear magnetic resonance c. Ion exchange chromatography & polyacrylamide gel electrophoresis d. Nuclear magnetic resonance & X-ray crystallography
i) Write down the equation derived from your Excel generated standard curve and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision iii) state the answer in a full sentence. (note: avoid mathematical symbols [=, +, -, etc] in your answer sentence).

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