Q: Why is Hydrogen peroxide solution so effective against anaerobes?
A: Hydrogen peroxide is a chemical compound that is acts as mild antiseptic. It is also an endogenous…
Q: Why do you have to boil the agar solution before dispensing it into tubes?
A: Agar is the medium which can used to grow the micro organisms in petri plates. The selected…
Q: How does colony morphology help you monitor the purity of your culture? What are disinfectants and…
A: Microbiology is the study of those living organisms which cannot be seen through naked eyes…
Q: What purpose does water serve in the “Effects of Antiseptics & Disinfectants”?
A: Answer: ANTISEPTICS : These are the liquid or semi-solid cream having some special properties to…
Q: What is the differece between disinfection and sterilization? What is the dfference between…
A: Disinfection and sterilization are essential for ensuring that medical and surgical instruments do…
Q: Is UVC disinfection of N95 face masks effective? Give two (2) important considerations for UVC…
A: Decontaminating masks could be effective against the shortage of masks in pandemics.UVC…
Q: What is the purpose of degerming? Does it completely eliminate microbes? What are some factors that…
A: 1.Degerming is the process used to reduce mircrobial numbers by gently scrubbing tissue with a mild…
Q: What is differential medium and differential media? Explain the difference ?
A: Difference between differential medumium - .- A differential medium support the expansion of any…
Q: why do microorganisms differ in their response to disinfectants
A: Microorganisms are the minute microscopic organism that is generally invisible to the human eye.…
Q: What are the advantages of UV disinfection versus, or as acomplement to, chemical disinfection with…
A: UV disinfection treatment is a very rapid physical process for disinfecting or sterilizing any…
Q: What is the purpose of food irradiation, and how does it work?
A: Irradiating food kills bacteria and extends life of the food.
Q: What is the difference between cleaning, sanitizing, and disinfecting?
A: Cleaning, sanitization and disinfection are the process of making an area free from all the visible…
Q: Is the autoclaving process absolute in killing all microorganisms? If not, what are the factors that…
A: Autoclave is an instrument or device which is used in method of moist heat sterilisation; while…
Q: If the inoculation of yeast during the process of vinegar making are not done, can we still produce…
A: Vinegar is nothing but acetic acid which is present in aqueous state. This can be used as flavouring…
Q: You perform a nitrate test. After adding solutions A and B, the broth remains colorless. Why should…
A: Nitrate test helps us to determine the production of nitrate reductase enzyme. By the activity of…
Q: If a component is heat sensitive, how might you conveniently achieve sterility of that medium? a.…
A: Option A i.e. filtration
Q: Why must you first clean debris from items before beginning disinfection or sterilization? (
A: Until the discovery of the microbes by the famous scientist Louis Pasteur, one of the signs of the…
Q: List one method of disinfection, and one method of sterilization. Label which is which.
A: Disinfection is a process by which most of the harmful microbes are killed or inactivated. However…
Q: Match the mode of disinfectant action for each of the following Gluteraidehydes Alcohol Phenols…
A: Here we are given mechanism of action of some disinfectants and i have matched them with their…
Q: How does an autoclave sterilize instruments? What is used to dry glassware and at the same time
A: Autoclaving is the best method for sterilizing glass instruments. Sterilizing means removal of all…
Q: Why can milk become sour due to bacteria even though it has been pasteurized?
A: Pasteurization is the process in which non-packaged and packaged foods such as fruit juice, milk, or…
Q: Agar is a complex polysaccharide obtained from seaweed and cannot be digested by most…
A: Growth media is the nutrients rich medium that helps in the growth of several bacteria and other…
Q: What is the most difficult type of microbial cell or structure to kill? What methods can be used to…
A:
Q: Which of the following control agents help to be used to achieve sterility? - Virucide - Sporocide…
A: Sterilization can be achieved through various means, including heat, chemicals, irradiation, high…
Q: How is household antibacterial soap like a weapon against pathogenic bacteria?
A: Antibacterial soaps are those that contains certain chemicals that inhibit the growth of pathogens .
Q: Why is it necessary to store the molten agar in a water bath at 50 degrees Celsius?
A: Introduction:- Agar is made up of two polysaccharides: agarose and agaropectin, with agarose…
Q: If my cultures are suffering severely due to alkaloid production and accumulation in the medium. In…
A: Alkaloids are basically the natural substances that can be used largely for human medication.…
Q: Explain why it is important to wash the wells with PBS between the additions of each reagent?
A: ELISA is a enzyme linked immunosorbent eassay. It is a process to get the measurements of antigens…
Q: Why is egg enrichment not added to BPA prior to sterilization?
A: Baird–parker agar or BPA is a selective medium is identify or isolate S. aureus in food. It contains…
Q: Why does hydrogen peroxide are most effective against probiotics?
A: Hydrogen peroxide is a peroxide. It acts as an oxidizing agent
Q: Which dye will not kill microorganisms if a wet preparation is made? Why?
A: Microorganisms are defined as organisms that cannot be viewed by the unaided eyes and are studied…
Q: What is the purpose of the different types of media (solid, semi-solid, broth)?
A: Culture medium is the medium consist of mixture of nutrients and substances which are required for…
Q: How might the physical differences between gram-positive and gram-negative bacteria contribute to…
A: Gram staining classifies bacteria into two groups based on their cell walls, namely Gram-positive…
Q: What are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one? Name…
A: Q)What are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one?…
Q: Why did we add agar after we measure the pH?
A: pH means p stands for potential or power.H stands for Hydrogen atom.It is the power of the Hydrogen…
Q: Is Vinegar a disinfectant?
A: It is not an EPA-registered disinfectant that means it won't kill 99.9 percent of bacteria and…
Q: How would you determine if a disinfectant or antiseptic is bactericidal or bacteriostatic
A: A bacteriocide is a chemical substance that kills bacteria. They can be disinfectants, antibiotics,…
Q: Why do petroleum-degrading bacteria need to attach to thesurface of oil droplets?
A: Petroleum is a crucial strategic resource and all countries compete fiercely for it. The…
Q: List 5 other methods of sterilization and describe the principle/s and when each method is used.
A: (Please note that, you provided more than one question, I can only answer one question at a time,…
Q: What is the difference between the use of Peppermint oil and Cinnamon oil in Veterinary Medicine to…
A: Need to find the difference in use of using peppermint and cinnamon oil in medicine to treated…
Q: Which piece of equipment sterilizes hospital tools and devices and how does it sterilize things? Are…
A: Sterilizing pieces of equipment are very important in modern healthcare settings. It is necessary…
Q: What is the major difference between Thayer-Martin and chocolate agar? When would you use…
A:
Q: What is the difference between a disinfectant and an antiseptic?
A: As per our company's guidelines we are supposed to answer single question at a time, please repost…
Q: what practical importance are air borne microorganisms to the laboratory workers? What precautions…
A: Laboratories are the areas where lot of experiments and research is performed. It deals with lot of…
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- The number of bacteria in saliva samples was determined by collecting the saliva, making serial dilutions, and inoculating nutrient agar by the pour plate method. The plates were inoculated aerobically for 48 hours at 37°C. What can you conclude from this data? Why? Mouthwash CFU per ml in salvia Before Use After Use Listerine 2.22 x 107 1.22x107 Schmidts Mouthwash 2.42 x 107 3.22x105 Biotene Mouthwash 3.21 x 107 2.12 x106Complete the table below with the observations: include amount of growth (-, +, ++, +, ++), colonial morphology for NA, color of colonies for EMB, and media color for MSA. Organism/ Media Nutrient Agar (NA) Eosin Methylene Blue (EMB) Agar Mannitol Salt Agar (MSA) Escherichia coli Pseudomonas aeruginosa Staphylococcus epidermidis Staphylococcus aureus Unknown 9 mixtureBelow is a scenario requiring the use of laboratory equipment and instruments. A watery fecal sample is tested for the presence of Bacillus cereus and Vibrio cholerae. The sample is stored in a screw capped tube. You are tasked to grow the Bacillus in a solid medium while Vibrio in a liquid medium. Choices: A. Incubator, B Hanging Drop Slide , C.Petri Dish, D. Drying Oven, E. Bunsen Burner, F. Inoculating NeedlE , G. Volumetric Pipette, H. Screw-capped Tubes, I. Hot plate, J. Centrifuge, K. Inoculating Loop, L. Microscope, M. Beaker, N. Biosafety Cabinet 1. What could be used to determine if there are more bacteria in the sample aside from Bacillus and Vibrio? 2. What should you use if you want to observe the natural motility of Bacillus under a Brightfield microscope? 3. Where should you perform the task of sterilization and inoculation? 4.What should be used to transfer the sample to the broth? 5. What could be used to separate the solid from the liquid portion of the…
- Based on the following image, which concentration of antibiotic would be considered the minimum bactericidal concentration (MBC)? a. 8 ug/mlb. 16 ug/mlc. 25 ug/mlExplain the following tests using Pseudomonas aeruginosa. Describe the purpose, reagents used and the results using Pseudomonas aeruginosa.EXPLAIN THE PURPOSE OF THE METHYL RED AND VOGES-PROSKAUER TESTS IN DETERMINING THE IDENTITY OF GRAM NEGATIVE ORGANISMS IN THE FAMILY ENTEROBACTERIACEAE. INCLUDE IN YOUR ANSWER WHY METHYL RED IS USED RATHER THAN PHENOL RED. WHAT ORGANISM IN THE FAMILY IS USED AS A CONTROL WHEN RUNNING THE METHYL TEST. IF THE METHYL RED TEST IS NEGATIVE, EXPLAIN WHY THE VOGES-PROSKAUER TEST IS RUN AND INCLUDE WHAT COLOR CHANGE INDICATES A POSITIVE TEST. ACCOUNT FOR THE COLOR DIFFERENCES IN THE NEGATIVE VOGES-PROSKAURER TESTS. WHAT REAGENTS (COMMON NAME) WERE USED IN THE VOGES -PROSKAUER TEST.
- Within a 3-day period at a large hospital, five patients undergoing hemodialysis developed fever and chills. Pseudomonas aeruginosa and Klebsiella pnuemoniae were isolated from three of the patients. P. aeruginosa, K. pneumoniae, and Enterobacter agglomerans were isolated from the dialysis system. Why do all three bacteria cause similar symptoms?The Kirby-Bauer test examines the effectiveness of antibiotics and disinfectants or antiseptics on separately plated bacteria. Which of the following Zones of Inhibition (ZOI) measurements on a plate of Klebsiella pneumoniae shows the strongest inhibition of the bacterium's growth? a) Penicillin, 7 mm ZOI b) Actinomycin, 9 mm ZOI c) Tetracycline, 26 mm ZOI d) Chloramphenicol 2 mm ZOI e) Streptomycin, 23 mm ZOIDesign a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration. show solution
- A student inoculates Escherichia coli into two test tubes containing the same sterile liquid medium, except the first tube also contains a drop of a chemical disinfectant with an antimicrobial effect. After 24 hours of incubation, the first tube remains clear while the second tube becomes cloudy with bacteria. How would you go about determining if the chemical is bacteriostatic or bactericidal?Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration.Describe how each of the following would appear when grown on EMB agar: a. Escherichia coli b. Pseudomonas aeruginos