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which is likely to be more immunogenic
- A protein with a molecular weight of 30,000
A protein with a molecular weight of 150,000
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- The protein illustrated below was isolated from a superhuman being. Scientists theorized that it was the source of power of its power. Give the name of the protein using the three letter symbol.Predict the number of bands and apparent mol. wt. of the following proteins on SDS gels. 1. Immunoglobulin G in a non-reducing gel (no β-mercaptoethanol added in the sample solution) – the light chains have a molecular weight of 25,000 Da and the heavy chains 50,000 Da.Predict the number of bands and apparent mol. wt. of the following proteins on SDS gels. 1. Immunoglobulin G in a reducing gel (β-mercaptoethanol added in the sample solution) – the light chains have a molecular weight of 25,000 Da and the heavy chains 50,000 Da.
- The limit for G-200 beads is 5000-600,000. When you pass two proteins- Protein A (75, 000), Protein B (1,000,000), through these beads, the protein that will elute out last is – They both elute out at the same time. Protein B Protein AGive 2 structural features in each of the given proteins below that make them perform their biological roles effectively (structural features that directly make them effective in their respective biological roles). Explain how each structural feature directly contributes to proteins’ biological roles. hemoglobin immunoglobulinThe protein illustrated in the attached picture was isolated from a superhuman being. Scientists theorized that it was the source of power of its power. 1.Give the name of the protein using the one letter symbol.
- Why are protein samples denatured before running SDS PAGE?A. To allow separation based on chargedB. To be able to visualize the proteinC. To allow separation based on sizeD. To be able to load samples correctly on the SDS-PAGE gelThe following protein quantification utilizes Cu ions except fora. Lowryb. Biuretc.BradfordThe protein illustrated below was isolated from a superhuman being. Scientists theorized that it was the source of power of its power. a. Construct the sequence of the protein using the three letter symbol.
- Arginine may protect against cardiac risks is it: a. True b. FalseA mixture of five proteins are analyzed by MALDI-TOF. Their molecular weigths are 3 kD, 22 kD, 100 kD, 225 kD and 515 kD. A peak appears in the mass spectrum at 33.3 kD. Which protein most likely gave this peak?Certain antibodies have been shown to bind only to the folded structure oftheir target protein ligand; yet others will bind to both the folded and denatured states of the same protein ligand. Explain this observation.