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Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time (mins) Group 3 (Ampicillin) Group 3(Control) Absorbance(60onm)

Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time (mins) Group 3 (Ampicillin) Group 3(Control) Absorbance(60onm)

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Procedure A Add ul ampicillin to 50 of Take mixture Mix 5ml 3 ml Top up to 50ml. the overnight culture with 45 ml LB broth to + Add fresh the LB mixture analyse both OD600 Withdraw 3ml mixture to The mixture is incubated incubator at 37°c 200rpm then Measure every 15 mins. Stop until 75 mins. Overnight culture analyse (OD600)record as zero time-point. at shaked (E-coli + LB borth) Mix overnight culture Sml Take 3 ml Add 50 ul of mixture to No ampicilin ampicillin to the B (control) with 45 ml LB analyse the mixture broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until 75 mins. - analyse (OD600)record as zero time-point. - incubated at shaked incubator at 37°c 200rpm Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time (mins) Group 3 (Ampicillin) Group 3(Control) Absorbance(6oonm)
Transcribed Image Text:Procedure A Add ul ampicillin to 50 of Take mixture Mix 5ml 3 ml Top up to 50ml. the overnight culture with 45 ml LB broth to + Add fresh the LB mixture analyse both OD600 Withdraw 3ml mixture to The mixture is incubated incubator at 37°c 200rpm then Measure every 15 mins. Stop until 75 mins. Overnight culture analyse (OD600)record as zero time-point. at shaked (E-coli + LB borth) Mix overnight culture Sml Take 3 ml Add 50 ul of mixture to No ampicilin ampicillin to the B (control) with 45 ml LB analyse the mixture broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until 75 mins. - analyse (OD600)record as zero time-point. - incubated at shaked incubator at 37°c 200rpm Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time (mins) Group 3 (Ampicillin) Group 3(Control) Absorbance(6oonm)
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