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Why are integral proteins more difficult to study then peripheral, and why integral can only be isolated with strong deteargents.
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- What is protein denaturation? Give five agents that can denature proteins Is it possible for a denatured protein to revert into its native/original form? Please explainIs an SDS-PAGE gel, what characteristic is used to separate the proteins? Which proteins move quickly? Which ones move slowly?(a) The octapeptide AVGWRVKS was digested with the enzyme trypsin . Which method would be most appropriate for separating the products: ion-exchange or gelfiltration chromatography? Explain. (b) Suppose that the peptide was digested with chymotrypsin . What would be the optimal separation technique? Explain.
- Why are structural analogs of sugar molecules (such as Oseltamivir and zanamivir) effective in treatment of influenza-virus infection?What could be seen in the organic acid precipitation during the protein denaturation experiment if an equal volume of picric acid solution was added to an albumin solution in a test tube? What form would the solution take when it is made?What is the typical composition of Harris Hematoxylin Solution? Which cellular organelle was more visible after immersing the intestinal cells in hematoxylin?