What is the BEST glassware that could be used to hold the agar powder while weighing? After 24 hours, growth can be seen from your plates. If you want to put dyes on your sample, what rack is used?
Q: (a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside…
A: Introduction A bacterial smear is a small layer of bacteria that has been stained on a slide. A…
Q: what color would you expect for a gram positive microorganism for each incorrect mistakes while…
A: "Gram staining" is a technique used in microbiology. It is a method in which a bacterium can be…
Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: Can culture media be sterilized in the hot air sterilizer? Why or why not?
A: Hot air sterilization is a procedure in which the sterilization of the objects are done using hot…
Q: Explain why it is important to use only a small amount of bacteria when preparing a smear. What are…
A: Many laboratory techniques, including the Gram-stain, involve the creation of a smear. The goal of…
Q: Choose two from the following culture preservations that are best to use when only an ordinary…
A: Many laboratories and biological companies maintain large culture collection centres. These are…
Q: For a successful direct staining, one should A. use a minimal amount of water to speed up the…
A: Direct staining is the process to make slides using a single stain or dye.
Q: In gram staining, could colors other than red be used as a counterstain?
A: A counterstain is a stain with color contrasting to the principle stain, making the stained…
Q: Agar is a solidifying agent used in media preparation. What is its origin?
A: Agar gel is a polysaccharide which can become liquefied at temperatures ranges from 85°C to 90°C and…
Q: What is the physical appearance (solid, liquid, semi-solid) of following media: agar plate
A: The Agar plate is made from mixing agarose and a growth medium onto a petri dish. Agarose is in a…
Q: Does the image above represent a pure culture? Yes O No
A: Answer- No
Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…
A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…
Q: If any of the water sample plates had a lawn or too many colonies to count, what might you have…
A: A technique known as the standard plate count is used for estimation of the population density in…
Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…
A: Making a solution thinner or less dense is the procedure of dilution. Serial dilutions (also known…
Q: Why after a certain period the growth rate of the culture decreases until growth stops?
A: Warmth, dampness, pH levels and oxygen levels are the four major physical and chemical components…
Q: a) explain in your own words how to perform specialized media tests to find an unknown bacteria
A: Different bacteria can be identified using the macroscopic properties of microbial cultures. The…
Q: What do to do when the disstillation did not make the sample completely pure? What could have went…
A: Distillation is a process that is based upon the boiling points of various liquids present in the…
Q: Put the numbers in the space provided to indicate the order of Capsule Staining. If the statement is…
A: The primary function of capsule staining is to differentiate capsular material from bacterial cells.…
Q: During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture…
A: Capsule stain is a type of differential stain which uses acidic and basic dyes to stain the…
Q: If you have a substance that you wish to sterilize (without destroying), and that substance is…
A: Introduction Any procedure that eliminates, eradicates, or renders inactive all life…
Q: bacteria (Mustard + 0.5% acetic acid or Mustard +1% acetic acid)
A: Different methods are used to prevent food from spoilage without changing its nutritional…
Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…
A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…
Q: You saw no color change in both the first step and second step of the Nitrate Reduction test. What…
A: A nitrate reduction test is a test done to test whether the Enterobacteriaceae member produces the…
Q: How is the correct way of incubating culture plate media? Explain why is that so.
A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…
Q: Agar is superior to gelatin as a solidifying agent because agar a. does not melt at room temperature…
A: Agar is used as a medium for culturing in laboratories.
Q: Solid media is usually prepared with a 1-2% final concentration of agar. If these plates were…
A: Solid media is the type of media that is made up of liquid substances like distilled water and other…
Q: You have a broth medium that you would like to turn into a solid medium in order to do streak…
A: The "nutrient broth" is essentially nutrient agar without the solidifying agent. They stay liquid at…
Q: Which of the following is the proper technique for inoculating an agar slant with a broth culture?…
A: If we want to grow microbes under lab conditions , we need to provide the necessary nutrients and…
Q: What is one advantage of a pour plate over a streak plate?
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: What would your final dilution be if you transferred 0.1 mL of a 10-° dilution to a sterile petri…
A: Dilution is a process by which solution (water or diluents) are added to the solvent (main solution)…
Q: chemically defined media b. complex culture media
A: Media is a growth medium of solid,liquid and semi solid type. Diffarent types of media are used for…
Q: When labeling culture tubes or plates why do we use a pencil instead of a pen? Are there other…
A: Appropriately labeling specimens for research labs is of critical importance.
Q: What is the advantage of the plating method over an electronic cell counting method in counting…
A: Bacteria culturing is done in order to grow the bacterial strains that are able to show resistance…
Q: You perform a nitrate test. After adding solutions A and B, the broth remains colorless. Why should…
A: Nitrate test helps us to determine the production of nitrate reductase enzyme. By the activity of…
Q: Make an accurate colored of what you observed on the mannitol salt agar plate.
A: Mannitol salt agar or MSA is a commonly used selective and differential growth medium in…
Q: In the preparation of a pure culture media, the petri plates are inverted when placed in an…
A: In microbiology, culture media is considered the growth medium that possesses the specific mixtures…
Q: Can culture media be sterilized in the hot air oven? why or why not?
A: Sterilization is the process of deactivating, killing, or removing any kind of microorganism (or…
Q: Why is a Bunsen burner used in the microbiology lab? (select all that apply) The flame is used to…
A: Answer -The flame is used to sterilize the inoculating loop. -The flame kills microorganisms that…
Q: With liquid media such as broth, is it possible to determine if the culture is pure using the naked…
A: A culture is a method by which microbes are allowed to reproduce and multiply in predetermined…
Q: What is the best streaking technique in a culture media? Explain
A: In microbiology, there are five I's that symbolise five different procedures used in the laboratory…
Q: what is usefulness of columbia CNA agar? Can it be used to test water ?
A: Columbia Agar is a highly nutritious base medium The addition of the antimicrobial agents, colistin…
Q: Why is it necessary to store the molten agar in a water bath at 50 degrees Celsius?
A: Introduction:- Agar is made up of two polysaccharides: agarose and agaropectin, with agarose…
Q: Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine C.…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: What type of agar would you use to grow gram negative bacteria
A: Gram-negative bacteria are the bacteria that do not consist of the crystal violet stain and they are…
Q: Why should your solvent be evaporated from the sample placed on the sterile disk before you place it…
A: The antimicrobial activity of natural compounds like essential oils derived from plants can tested…
Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?
A: Introduction We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…
Q: What is the hazard of the spattering tendency in heating
A: Sterilization is the process of cleaning a substance by removing, killing or deactivating all forms…
Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…
A:
Q: What is the meaning of the word sterile? What would happen if culture media freshly prepared were…
A: Sterile It refers to the state of being free from any bacteria or any other living microorganisms.…
Q: Which ingredient is included in the motility test to make the results easier to see? bromthymol blue…
A: A motility test is a test that is performed to check whether the organism is motile in its nature or…
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- what effect would failing to drain the smear in-between staining have on your judgment of the gram reaction of an organism?Can culture media be sterilized in the hot air sterilizer? Why or why not?Which of the following can be used to distinguish one colony from another on an agar plate? Select all that apply. a. Shape b. Color c. Gram stain d. Cell arrangement e. Size f. Elevation g. Margin h. Texture
- During media preparation, you observed that the agar medium did not solidify after sterilization even after incubating it for 1 hour at room temperature. What could be the reason behind this?When labeling culture tubes or plates why do we use a pencil instead of a pen? Are there other alternatives in labeling?During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture is discarded in the disinfectant solution. Why?
- In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!What are pure cultures and why are they important? How are spread plates, streak plates, and pour plates prepared?
- Can culture media be placed in a freezer? Why or why not?You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a day