Q: What is the usual stain used for staining a sputum smear?
A: Essentially, sputum comprises of a combination of saliva and mucus. additionally named as phlegm, It…
Q: what is the procedure and materials you need to stain Staphylococcus aureus?
A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium hat belongs to the Firmicutes and…
Q: What is the purpose of fixing a smear?
A: Smear- a smear is a thin layer of a sample such as bacterial cells or blood spread evenly on a glass…
Q: Do you think UVC disinfection of N95 face masks is effective? Give two important considerations for…
A: At the time of Covid-19, N95 masks are currently considered critical supplies by the centers for…
Q: Why is it important to limit the quantity of cells used to prepare a smear?
A: A thin layer of cells or tissues that are taken from the body of an organism under study which is…
Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…
A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…
Q: Describe the Gram staining procedure
A: Gram staining is used to differentiate between Gram positive and Gram negative bacteria. These…
Q: Does the Gram stain provide information regarding a bacterium's susceptibility to antimicrobial…
A: The Gram stain is performed for the determination and classification of the bacteria. In Gram…
Q: Why bleach disinfectant will the most effective against probiotic?
A: Probiotics are live microorganisms that have health benefits when consumed. These are usually…
Q: What is the purpose of Thick smear? Purpose of Thin smear?
A: Blood smear:-Also known as peripheral blood smear. -Blood smear has many different uses ranging from…
Q: Give two reasons for heating the slide after the smear is air dried?
A: Smear is the thin film of the sample that is spread over the slide to be observed in the microscope.…
Q: Why is the Gram stain considered a differential stain?
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: In gram staining, could colors other than red be used as a counterstain?
A: A counterstain is a stain with color contrasting to the principle stain, making the stained…
Q: Is the flagellar stain a Positive or Negative Simple Stain protocol?
A: Staining is a technique that is used to enhance contrast in samples, generally at the microscopic…
Q: When bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times…
A: The initial identification of the streptococcal and enterococcal species is based on their hemolytic…
Q: Which microbe requires serum components to be added to the growth medium?
A: Some microbes are nutritionally fastidious and hence needs some substances to be added to the growth…
Q: describe the agar-overlay technique
A: Biological techniques are processes or methods for trying to study living things. They include…
Q: What is the significance of heat fixing the smear?
A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…
Q: Where should a label be written on an agar plate?
A: The aseptic technique is applied in a laboratory setup to reduce the risk of contamination. It…
Q: What is the difference between streak plate method and spread plate technique?
A: Answer of the question given below...
Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…
Q: I Why does't a negative stain colorize the cells in the smear?
A: Staining is a technique which is used for enhancement of contrast in samples mostly at the level of…
Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
Q: Why should culture media after inoculation be incubated at an optimal temperature immediately?
A: Culture media, also called as growth media, are particular combinations of nutrients and other…
Q: Explain why the media where the Staphylococcus aureus is plated has turned yellow but the media…
A: Mannitol Salt Agar (MSA) is a differential and selective medium. A member of the staphylococcus…
Q: Can you please help me describe the colony apperance on these two stains? Thank you! Growth on Blood…
A: The chocolate agar and the blood agar media both have the almost same composition. The only…
Q: Give at least two other examples of samples that are best prepared using Smear Preparation…
A: Smear preparation technique: it is used to fix the samples onto the slides.
Q: Which step of the gram stain procedure is most critical?why?
A: Gram staining is one of the most common laboratories test to identify the different bacterial…
Q: Explain why it is important to use only a small amount of bacteria when preparing a smear
A:
Q: What are the principle and basic concepts of SMEAR PREPARATION? (please explain it thoroughly in a…
A: Introduction A smear is a small amount of culture spread in a very thin film on the surface of the…
Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…
A: Preparation of culture media and inoculation are two of the very sensitive steps in any…
Q: purpose of using serum in culture media
A: A culture media is a media made in the lab, that is curated to facilitate the culture and growth of…
Q: What is selective medium?
A: Description of a selective medium.
Q: Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?
A: Gram staining is a differential staining technique developed by Danish bateriologist Hans Christian…
Q: is staphylococcus aureus cells under 40 times magnification bigger than onion cell or cheek cell?
A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium that belongs to the Firmicutes and…
Q: Is UV treament more or less effective than disinfection when used against Saphylococcus aureus?
A: Saphylococcus aureus is a gram-positive bacterium that is found in the human nose, skin, and…
Q: Why is 15 minutes generally used for sterilization? Why is agar added to medium?
A: Sterilization is the process in which all life forms like microbes (fungi, bacteria, viruses,…
Q: How can moist heat sterilization can sterilize antibiotic solution?
A: Moist heat sterilization is a technique that uses heated, increased pressure steam to sterilize an…
Q: if there are features of the gram stain that are not as expected what possible sources or error may…
A: Gram staining is a general technique that is used to differentiate two larger groups of bacteria…
Q: microbial sensivity lab: in the procedure used to test bacterial growth against various temperatures…
A: Growth of bacteria differs with varying temperature. Bacteria grows rapidly in the temperature range…
Q: How does the agar dilution method differ from streaking toobtain isolated colonies?
A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…
Q: What are the advantages and disadvantages of using the Slide Culture technique?
A: Slide culture technique is mainly utilized for the preparation of fugal colonies. Fungi are…
Q: How is the Gram stain used clinically to help identify the cause of an infection?
A: Gram stain or sometimes "Gram staining", likewise called Gram's method, is a technique for staining…
Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…
A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…
Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…
A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.
Q: Why should freshly collected samples be used for microscopic examination?
A: The benefit of the microscope is that it produces a better resolution than the naked eye. The higher…
Q: What is meant by the word sterile? Why is aseptic techniquenecessary for successful maintenance of…
A: Microbes are all pervading. They are found everywhere, in air, water, any substratum, inside and on…
Q: How can the thickness of the smear affect the staining procedure?
A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…
Q: What is the purpose of using the streak plate method?
A: Solution- Introduction - Streak plate technique is used for the isolation into a pure culture of…
Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…
A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…
Why is it important to limit the quantity of cells used to prepare a smear?
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