Why might it be very difficult to visualize the separation of cis- and trans-2-butene by TLC?

Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.

How does increasing the polarity of the developing solvent affect the Rf of a compound on a silica gel TLC plate?

Why mixture of solvents such as ethyl acetate with 5% acetic acid is used as a solvent/mobile phase for developing TLC? Please explain in detail.

Why is GSC not used nearly as extensively as GLC?

What is the order in which the following compounds would he eluted from an HPLC column containing a reversed -phase packing?(a) benzene, diethyl ether, n-hexane(b) acetone, dichloroethane, acetamide

You will run a TLC plate after the CC placing in one spot the original sample of pigments, one spot from the yellow band and one from the green band. Draw a picture of what you expect to observe after running the TLC plate. Explain.

I need help darwing a TLC plate  and figuring out the Rf values is the retention factor values We estimate our own TLC plate of Anthracene, Maleic Anhydride, and the Diels-Alder Adduct and determine the Rf values for the three materials

In order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc,)

Why do the ‘% Area’ values on the HPLC not correspond directly to composition by mole?