Would you say that the GadC anti porter exhibits the same pH dependence as the AdiC anti porter? If not which anti porter is less active at nonacid pHs?
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Q: C
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Would you say that the GadC anti porter exhibits the same pH dependence as the AdiC anti porter? If not which anti porter is less active at nonacid pHs?
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- When comparing the structures of acetate buffer and tris-maleate buffer, acetate buffer works on fumarase activity but tris-maleate buffer does not?What is kremil-s for and What's the relation of kremil-s to biology? What is the meaning behind the increase in number of water refilling stations in a particular place?The GadC anti porter also transports the amino acid glutamine(Gln). Do you think this activity has any role to play in combating low ph environments? How would you test this hypothesis?
- Based on our pka's table, pKa1 of -COOH group of Cys = 1.71 pKa2 of -NH2 group of Cys = 10.78 pKa3 of side chain group of Cys = 8.33 If pKa is different, will the buffer change??Determine the net charge of the predominant form of Arg at (a) pH 1.0, (b) pH 5.0, (c) pH 10.5, and (d) pH 13.5.Acetazolamide is a carbonic anhydrase inhibitor that is used to treat glaucoma. It has two acidic groups and has 2 pKb’s as shown below: What is the concentration of acetazolamide (B), in a 0.5000 M solution of acetazolamide (B)? What is the concentration of hydrogen acetazolamide (BH+) in a 0.5000 M solution of acetazolamide (B)?
- When eluting your analyte of interest using HPLC, gradient elution can be used. How does Gradient elution differ from Isocratic and comment on the advantages of using this type of mobile phase elution?Is the glutamate-GABA anti porter GadC active at nonacid pHs?The enzyme phospholipase A2 has an optimal pH of 5.5. If a biochemist wants to set up an in vitro assay to screen inhibitors of this enzyme, which buffer system would be BEST?
- Acetazolamide is a carbonic anhydrase inhibitor that is used to treat glaucoma. It has two acidic groups and has 2 pKb’s as shown below: What is the concentration of dihydrogen acetazolamide (BH22+) in a 0.5000 M solution of acetazolamide (B)? What is the concentration of hydronium ion (H+) in a 0.5000 M solution of acetazolamide (B)?The Bradford reagent gives a linear response only from 0.1 mg/mL to 1.4 mg/mL of protein concentration. Would we be able to use an "absorbance vs. concentration" line graph for samples outside of the range indicated? Justify your response.How was thermal stability measured in this paper and describe how GRL-1720 and 5h affect the thermal stability of Mpro and which drug has the larger effect? https://www.nature.com/articles/s41467-021-20900-6