You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin C-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolated from pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purified mRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube contains purified mRNA isolated from pancreatic β-cells from a patient during absorptive state. In not more than 2 pages (using 1.5 line space of Arial or Times New Roman fonts) provide answers for the following questions? 1) Describe the primary procedure (key steps no details) that you will follow to clone the C-peptide gene from the RNA above into the only vector you have, a pUC expression vector cut open using EcoR1 which has a 5-recognition site and a 3- HindIII in the MCS?
You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin
C-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolated
from pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purified
mRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube contains
purified mRNA isolated from pancreatic β-cells from a patient during absorptive state. In not more
than 2 pages (using 1.5 line space of Arial or Times New Roman fonts) provide answers for the
following questions?
1) Describe the primary procedure (key steps no details) that you will follow to clone the C-peptide gene
from the RNA above into the only vector you have, a pUC expression vector cut open using EcoR1 which
has a 5-recognition site and a 3- HindIII in the MCS?
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