You have completed an in vitro mutagenesis experiment to create an G to Tmutation in the coding sequence of the heat shock protein gene. This gene is cloned in a plasmid and you want to confirm that the mutation was correctly introduced. Which scquencing technolsogy be most appropriate to use practically and cconomically. Microarray Sanger sequencing RNA sequencing Exon sequencing

You have completed an in vitro mutagenesis experiment to create an G to Tmutation in the coding sequence of the heat shock protein gene. This gene is cloned in a plasmid and you want to confirm that the mutation was correctly introduced. Which scquencing technolsogy be most appropriate to use practically and cconomically. Microarray Sanger sequencing RNA sequencing Exon sequencing

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter14: Dna Structure And Function

Section: Chapter Questions

Problem 37CTQ: An adult with a history of tanning has his genome sequenced. The beginning of a protein-coding...

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Briefly discuss the process of gene therapy, including some of its technical challenges.
Transcribe and translate the following DNA sequence (nontemplate strand): 5'- ATGGCCGGTTATTAAGCA-3'
A scientist randomly mutates the DNA of a bacterium. She then sequences the bacterium’s daughter cells, and finds that the daughters have many errors in their replicated DNA. The parent bacterium likely acquired a mutation in which enzyme? DNA ligase DNA pol II Primase DNA pol I
An adult with a history of tanning has his genome sequenced. The beginning of a protein-coding region of his DNA reads ATGGGGATATGGCAT. If the protein-coding region of a healthy adult reads ATGGGGATATGAGCAT, identify the site and type of mutation.
2. You are cloning the genome of a new DNA virus into pUC18. You plate out your transformants on ampicillin plates con-taining X-gal and pick one blue colony and one white colony. When you check the size of the inserts in each plasmid (blueand white), you are surprised to fi nd that the plasmid fromthe blue colony contains a very small insert of approximately60 bp, while the plasmid from the white colony does notappear to contain any insert at all. Explain these results.
15. A method of sequencing long DNA fragments by generating new sequencing primers based on successive DNA reads is called?
2AQ:You are cloning the genome of a new DNA virus into pUC18.You plate out your transformants on ampicillin plates containing X-gal and pick one bluecolony and one white colony. When you check the size of the inserts in each plasmid (blueand white), you are surprised to find that the plasmid from the blue colony contains a verysmall insert of approximately 60 bp, while the plasmid from the white colony does notappear to contain any insert at all. Explain these results.
35) A researcher inserted DNA fragments from an organism into expression vector plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be an effective means of identifying which clones contain a specific gene of interest?
8. Using recombinant DNA techniques (which willbe described in Chapter 9), it is possible to take theDNA of a gene from any source and place it on achromosome in the nucleus of a yeast cell. Whenyou take the DNA for a human gene and put it into ayeast cell chromosome, the altered yeast cell canmake the human protein. But when you remove theDNA for a gene normally present on yeast mitochondrial chromosomes and put it on a yeast chromosome inthe nucleus, the yeast cell cannot synthesize the correctprotein, even though the gene comes from the sameorganism. Explain. What would you need to do to ensurethat such a yeast cell could make the correct protein?
39. Which of the following are general features found in a plasmid used for bacterial cloning? Group of answer choices Origin of replication Multiple cloning site Multiple cloning site and antibiotic resistance gene Multiple cloning site, antibiotic resistance gene, and origin of replication Locus of transformation Locus of transformation, multiple cloning site, and antibiotic resistance gene Antibiotic resistance gene
35. What is the purpose of the beta-galactosidase gene found in many plasmids for cloning? Group of answer choices It allows for colored screening of bacterial cells as a selection step. The expressed protein is important for the metabolism of the bacteria. It is used as a control for sequencing It is involved in the replication processes when the plasmid is copied inside the cell. The gene allows for higher transformation efficiencies.
27. You have a DNA sequence of 1400 bp. To characterize it, you decide to make a restriction map. Cutting with the first enzyme, you show by electrophoresis that the fragments produced are 400 and 1000 bp, respectively. After using the second enzyme, you recover 600-bp and 800-bp fragments. After treatment with both enzymes, fragments were 200, 400, and 800 bp. Draw the correct restriction map.