You have completed an in vitro mutagenesis experiment to create an G to T mutation in the coding sequence of the heat shock protein gene. This gene is cloned in a plasmid and you want to confirm that the mutation was correctly introduced. Which sequencing technology be most.appropriate to use practically and sconomically: Microarray O Sanger sequencing RNA sequencing Exon sequencing

You have completed an in vitro mutagenesis experiment to create an G to T mutation in the coding sequence of the heat shock protein gene. This gene is cloned in a plasmid and you want to confirm that the mutation was correctly introduced. Which sequencing technology be most.appropriate to use practically and sconomically: Microarray O Sanger sequencing RNA sequencing Exon sequencing

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter14: Dna Structure And Function

Section: Chapter Questions

Problem 37CTQ: An adult with a history of tanning has his genome sequenced. The beginning of a protein-coding...

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2. You are cloning the genome of a new DNA virus into pUC18. You plate out your transformants on ampicillin plates con-taining X-gal and pick one blue colony and one white colony. When you check the size of the inserts in each plasmid (blueand white), you are surprised to fi nd that the plasmid fromthe blue colony contains a very small insert of approximately60 bp, while the plasmid from the white colony does notappear to contain any insert at all. Explain these results.
15. A method of sequencing long DNA fragments by generating new sequencing primers based on successive DNA reads is called?
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2AQ:You are cloning the genome of a new DNA virus into pUC18.You plate out your transformants on ampicillin plates containing X-gal and pick one bluecolony and one white colony. When you check the size of the inserts in each plasmid (blueand white), you are surprised to find that the plasmid from the blue colony contains a verysmall insert of approximately 60 bp, while the plasmid from the white colony does notappear to contain any insert at all. Explain these results.
39. Which of the following are general features found in a plasmid used for bacterial cloning? Group of answer choices Origin of replication Multiple cloning site Multiple cloning site and antibiotic resistance gene Multiple cloning site, antibiotic resistance gene, and origin of replication Locus of transformation Locus of transformation, multiple cloning site, and antibiotic resistance gene Antibiotic resistance gene
You are cloning the genome of a new DNA virus into pUC18.You plate out your transformants on ampicillin plates containing X-gal and pick one blue colony and one white colony.When you check the size of the inserts in each plasmid (blueand white), you are surprised to fi nd that the plasmid fromthe blue colony contains a very small insert of approximately60 bp, while the plasmid from the white colony does notappear to contain any insert at all. Explain these results.
35. What is the purpose of the beta-galactosidase gene found in many plasmids for cloning? Group of answer choices It allows for colored screening of bacterial cells as a selection step. The expressed protein is important for the metabolism of the bacteria. It is used as a control for sequencing It is involved in the replication processes when the plasmid is copied inside the cell. The gene allows for higher transformation efficiencies.