You have discovered an enzyme secreted by a particularly virulent bacterium that cleaves the C2′—C3′ bond in the deoxyribose residues of duplex DNA. What is the eff ect of this enzyme on supercoiled DNA?

Q: Prokaryotic DNA is normally slightly negatively supercoiled, which can lead to "DNA breathing". How…

A: DNA breathing is defined as the short and temporary or in other words a transient opening of nucleic…

Q: What is the completing sequence of nitrogen- containing bases for a AGCCGTTAAC fragment of a DNA…

A: DNA base pairs are due to the formation of hydrogen bonds between the nitrogenase bases, purines,…

Q: In a DNA double helix, why doesn't an A or T form two hydrogen bonds (out of three possible) with G…

A: The DNA (deoxyribonucleic acid) is made up of nucleotide bases that are made up of nitrogenous bases…

Q: Why are most DNA-binding proteins specific to certain chemicalgroups within the DNA?

A: DNA is the genetic material that carries genetic information in the form of coded nucleotide…

Q: in the DNA of certain bacterial cells, 13% of the nucleotides are adenine. What are the percentages…

A: Deoxyribonucleic Acid (DNA) is a double-stranded,  right-handed helical molecule that is twisted…

Q: What is the nucleotide sequence of the complementary strand of the DNA molecule:…

A: Nucleic acids are large macromolecules that store hereditary information for cellular growth and…

Q: The DNA of a deletion of alpha bacteriophage has a length of 15 micrometers instead of 17…

A: The bacteriophage is those viruses that have the capability to infect bacteria and reproduce inside…

Q: Explain why the bending of the double helix to form a superhelix falls into the area of geometry or…

A: Nucleic acids are important biomolecules. Nucleic acids are of two types. These are DNA…

Q: You have determined that the newly synthesized strand of DNA in your sequencing reaction has the…

A: Template Strand will be: 3' TGATGGCTCA5'

Q: What is the complete base composition of a double- stranded eukaryotic DNA that contains 22%…

A: the 4 nucleotides that make up the DNA show a unique property of complementary base pairing wherein…

Q: What entropic factor destabilizes helical DNA at high temperature?

A: The double (ds) stranded DNA undergoes structural changes also known as denaturation at high…

Q: Which of the following (A through E ) would you expect to find in a nucleotide chain constructed for…

A: Sanger sequencing is the method developed to sequence the short sequence of nucleotides base pair in…

Q: DNA isolated from the bacterial virus M13 contains 25% A, 33% T, 22% C, and 20% G. Do these results…

A: DNA  is deoxyribose nucleic acid it contains the nitrogenous bases of adenine guanine thymine and…

Q: A closed circular duplex DNA has a 100-bp segment of alternating C and G residues. On transfer to a…

A: B-DNA IS BIOLOGICALLY THE MOST COMMON –RIGHT-HANDED (20 ANGSTROM (A) DIAMETER) –COMPLEMENTARY…

Q: What is an Okazaki fragment? In which strand of replicating DNA are Okazaki fragments found? Based…

A: DNA (Deoxyribonucleic acid) is the genetic material, which gets copied during cell division. The…

Q: Draw the syn conformation of purine residues in Z-DNA.

A:

Q: Given the structures of the nitrogenous bases shown in the picture, draw the structure of a part of…

A: The nitrogenous bases shown in the figure are Adenine (A), Guanine (G), cytosine (C), uracil (U) and…

Q: You prepare a reaction mix containing (i) DNA polymerase II, (ii) DATP, dCTP, DGTP, Mg2+, and…

A: DNA replication is considered a process, during which new strands are synthesized.

Q: The base analog 5-bromouracil (5BU), which sterically resembles thymine, more readily undergoes…

A: Base analogs are the purines and pyrimidines that are similar in structure to the normal DNA bases.…

Q: What is the name of the enzyme that add nucleotides to a growing DNA strand? What kind of bonds…

A: DNA strands are essentially polymers that comprise of deoxynucleoside monophosphate that are linked…

Q: Name and write the structures of the purine and pyrimidine bases present in DNA.

A: DNA is a polynucleotide strand. DNA is transcribed into RNA and RNA is translated into proteins.…

Q: What is the concentration of a DNA solution that absorbs 0.812 and 0.463 at 260 and 280 nm,…

A: Deoxyribonucleic acid (DNA) is the genetic material. It is polymer of nucleotides. The protein…

Q: Insulin is synthesized as preproinsulin, which has 81 amino acids. How many heterocyclic bases must…

A: Insulin is synthesized from preproinsulin. The preprotein is then cleaved by a proteolytic enzyme to…

Q: What is the real definition of DNA ligase to make it true

A: In DNA replication, an enzyme called DNA ligase is used  during cell division.  After the primer is…

Q: Why do you think the first part of DNA (deoxyribonucleic acid) is different from RNA (Ribonucleic…

A: DNA or Deoxyribonucleic Acid is a specific molecule which comprises of two polynucleotide chains.…

Q: Kornberg showed that nucleotides are added to the 3' end of eachgrowing DNA strand. In what way does…

A: It is majorly present within the nucleus. DNA can exist in different levels of complexity.  Primary…

Q: If one strand of a DNA molecule has the base sequence 5′-AGCATTAAGCT-3′, what is the base sequence…

A: The complementary base pairing rule or Chargaff’s rule states that the DNA base pairs are always…

Q: What part(s) of a nucleotide (namely, phosphate, sugar, and/orbase) is(are) found in the major and…

A: Nucleotides are composed of a phosphate group, a pentose sugar, and a nitrogenous base. The pentose…

Q: Why is it necessary for naturally occurring DNA molecules to be negatively supercoiled?

A: DNA supercoiling is important for DNA packaging within all cells.

Q: Why is a methylated base employed in DNA and not in RNA?

A: DNA and RNA are the two types of nucleic acids. Deoxyribose nucleic acid (DNA) is present in all…

Q: Why does DNA with a high A-T content have a lower melting temperature, Tm, than DNA with a high G-C…

A: Deoxyribonucleic acid(DNA) is a molecule that contains two polynucleotide chains that coil around…

Q: Draw deoxyribonucleosides in the conformation they would appear in Z-DNA and B-DNA.

A: Z DNA

Q: Escherichia coli and other bacteria methylate adenines on the original strand to distinguish the…

A: When growing a cell divide, they need to copy their genetic material and distribute it to ensure…

Q: Extrachromosomal DNA is critical to the antibiotic resistance found in microorganisms, how do these…

A: Extrachromosomal DNA means a DNA which is present independently of the main DNA or chromosome.…

Q: Which positions in the purine ring of a purine nucleotide in DNAhave the potential to form hydrogen…

A: DNA is the chemical name for the molecule that carries genetic instruction is all living things. The…

Q: The backbone of the DNA molecule is made up of two alternating componebts, what are these? Explain.

A: DNA is considered to be the genetic material within the nucleus that gets packed in thread-like…

Q: You add 1 ul of EcoRI into 50 ul of your reaction of which contains 10 ug of DNA. How long would it…

A: 1 hr

Q: Which of the following DNA strands below is expected to have the highest melting temperature?

A: DNA is the genetic material in living beings. It can be seen both in prokaryotes and eukaryotes. The…

Q: What are the bonds that are broken during the chemical reaction that results from the activity of…

A: There are two types of bonds present in the DNA double helix i.e. covalent and hydrogen. Covalent…

Q: When an aromatic hydrocarbon intercalates between two stacked base pairs, what effect may there be…

A: DNA is made up of bases such as guanine, adenine, cytosine, and thymine. The canonical DNA present…

Q: In the DNA double-helix structure, the larger of the two grooves formed by the helical twist where…

A: DNA is composed of nucleotides which form its building blocks. Each nucleotide comprises a…

Q: Why is it not surprising that the addition of nucleotides to a growing DNA chain takes place by…

A: DNA replication, or copying of a cell's DNA, is a complicated task. There are around 3 billion base…

Q: Why do higher salt concentrations stabilize the DNA double helix? Or What aspect of the structure of…

A: DNA is the double helical structure in which both the strand are antiparallel to each other. The…

Q: In sea urchin DNA, which is double stranded, 17.5percent of the bases were shown to be cytosine (C).…

A: Chargaff's rule expresses that DNA from any organism ought to have a 1:1 stoichiometric proportion…

Q: If a region of one strand of a DNA double helix has the sequence 5'-CAGATAC-3', what is the sequence…

A: The nucleotide base pairing is complementary that is two complementary nitrogenous molecules are…

Nucleotides

It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid). 

Nucleic Acids

Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.