To explain: The effect of DNA sequencing reaction if the ratio of dideoxyribonucleoside triphosphates to deoxyribonucleoside triphosphates is increased.
Introduction: The laboratory technique used to determine the exact arrangement of the
To explain: The effect of DNA sequencing reaction if the ratio of dideoxyribonucleoside triphosphates to deoxyribonucleoside triphosphates is decreased.
Introduction: The laboratory technique used to determine the exact arrangement of the nucleotide bases [adenine (A), cytosine (C), thymine (T), and guanine (G)] in a portion of DNA molecule is called as DNA sequencing. They have a wide variety of applications in the field of medicine, forensics, and agriculture. They are used to identify the genes that can lead to cause many genetic diseases. Sanger sequencing is a type of DNA sequencing that involves the production of multiple copies of the target DNA. The ingredients used in this sequencing involve DNA polymerase enzyme, primers, dideoxyribonucleoside triphosphates (ddNTPs), deoxyribonucleoside triphosphates (dNTPs), and the template DNA to be sequenced.
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Essential Cell Biology (fifth Edition)
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