Brock Biology of Microorganisms
15th Edition
ISBN: 9780134626352
Author: MADIGAN
Publisher: PEARSON
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Chapter 11.9, Problem 1CR
Q What is a merodiploid and how does an F′ plasmid yield a merodiploid?
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For each of the pLM160 plasmid elements listed below: define the component AND briefly discuss the functional significance of this component for recombineering in Chlamydomonas reinhardtii.
a. mNeonGreen-3xFLAG
b. KanR2
c. 5’ cloning primer annealing site
What is the difference between a l insertion vector such aslgt11 and a l replacement vector? What is the advantage ofeach?
How many fragments will be formed after digestion in sample C, if the plasmid is circular and why?
How was RAD52 expression observed?
Chapter 11 Solutions
Brock Biology of Microorganisms
Ch. 11.1 - Distinguish between a mutation and a mutant.Ch. 11.1 - Distinguish between screening and selection.Ch. 11.1 - Prob. 3MQCh. 11.1 - Write a one-sentence definition of the term...Ch. 11.2 - Do missense mutations occur in genes encoding...Ch. 11.2 - Why do frameshift mutations generally have more...Ch. 11.2 - Prob. 1CRCh. 11.3 - Why are suppressor tRNA mutations not lethal?Ch. 11.3 - Which class of mutation, missense or nonsense, is...Ch. 11.3 - What is the difference between same-site and...
Ch. 11.4 - Prob. 1MQCh. 11.4 - Prob. 2MQCh. 11.4 - Prob. 1CRCh. 11.5 - Which protein, found in virtually all cells,...Ch. 11.5 - Explain the fate of transferred chromosomal DNA if...Ch. 11.5 - Prob. 3MQCh. 11.5 - What are heteroduplex regions of DNA and what...Ch. 11.6 - During transformation a cell usually incorporates...Ch. 11.6 - In genetic transformation, what is meant by the...Ch. 11.6 - QExplain why recipient cells do not successfully...Ch. 11.7 - Prob. 1MQCh. 11.7 - What is the major difference between generalized...Ch. 11.7 - Why is phage conversion considered beneficial to...Ch. 11.7 - QExplain how a generalized transducing particle...Ch. 11.8 - In conjugation, how are donor and recipient cells...Ch. 11.8 - Explain how rolling circle DNA replication allows...Ch. 11.8 - QWhat is a sex pilus and which cell type, F or F+,...Ch. 11.9 - In conjugation involving the F plasmid of...Ch. 11.9 - Prob. 2MQCh. 11.9 - Prob. 3MQCh. 11.9 - QWhat is a merodiploid and how does an F plasmid...Ch. 11.10 - Why is it usually more difficult to select...Ch. 11.10 - Why do penicillins not kill species of Archaea?Ch. 11.10 - Explain one type of conjugation in Archaea and how...Ch. 11.11 - Prob. 1MQCh. 11.11 - What is the significance of the terminal inverted...Ch. 11.11 - How can transposons be used in bacterial genetics?Ch. 11.11 - Prob. 1CRCh. 11.12 - Why is the CRISPR system considered a prokaryotic...Ch. 11.12 - Prob. 2MQCh. 11.12 - QExplain why incoming DNA recognized by a short...Ch. 11 - A constitutive mutant is a strain that...Ch. 11 - Although a large number of mutagenic chemicals are...Ch. 11 - Why is it difficult in a single experiment to...Ch. 11 - Prob. 4AQ
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- To identify mutant genes, a wild-type bacterial genomiclibrary in plasmids is used to transform the mutant bacterialstrain. A transformed ________ in which the mutantphenotype is rescued to wild type likely harbors a plasmidcontaining a wild-type copy of the corresponding genearrow_forwardExplain how to identify mutant genes molecularly bytransformation with recombinant plasmids.arrow_forward- Explain how you were able to identify plasmid in each sample using the gel, considering how linear DNA fragments of different length are separated by agarose gel electrophoresis.- Why do supercoiled, nicked and linear DNA sequences of the same size (kb) migrate at different rates during agarose gel electrophoresis?- How does SYBR Safe® enable the visualisation of the location of DNA fragments on the gel?arrow_forward
- Explain why digestion with EcoRI and Hindlll might give more than one insert fragment with some of the recombinant plasmidsarrow_forwardWe use a NanoDrop spectrophotometer to analyze plasmid DNA obtained from miniprep. Whatinformation about the plasmid does the 260nm reading alone provide and why?arrow_forwardIdentify mutant genes by plasmid library transformation?arrow_forward
- Briefly describe (in 6-7sentences) what a plasmid is, and how it can be used to transform bacteria like E. coli.arrow_forwardCalculate the purity of the plasmid DNA. Do you think the plasmid is pure? Why?arrow_forward1)Explain why there is a part of the Lacz gene in pUC18119 and also how the natural biochemical process wherein it normally functions is used during cloning by molecular biology. 2)Write down the basepairs of double-stranded ONA that is generated at the joint between a plasmid that is digested with Xhol and a DNA fragment that is digested with Sall and joined by DNA-ligase. Indicate the 5'- and 3'- termini of each strand.arrow_forward
- Describe briefly the nature of the genetic material 2. How might the knowledge in telomeres and telomerase be applied to anti-aging strategies? Are such strategies being developed?arrow_forwardWhat is o A vector derived from the tumor-inducing (Ti) plasmid of the bacterium Agrobacterium tumefaciens is the basis for an efficient method for introducing transgenes into plants?arrow_forwardHow is telomerases related to ageing? How Can telomerase- related ageing be addressed therapeutically?arrow_forward
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