Concept explainers
To explain: The Ura3 based selection identifies cells with defects in components required for import into the mitochondrial matrix.
Concept introduction: Ura 3 gene is involved in pyrimidine biosynthesis. Ura 3 gene produces an enzyme called Orotidine 5'-phosphate decarboxylase. Orotidine 5'-phosphate decarboxylase catalyses the decarboxylation step of orotidine monophosphate (OMP) to give uridine monophosphate(UMP) during pyrimidine biosynthesis. Uracil biosynthesis occurs in the cytosol.
To explain: The reason why the normal cells with modified Ura3 gene doesn’t grow in the absence of uracil.
Concept introduction: Ura 3 gene is involved in pyrimidine biosynthesis. Ura 3 gene produces an enzyme called Orotidine 5'-phosphate decarboxylase. Orotidine 5'-phosphate decarboxylase catalyses the decarboxylation step of orotidine monophosphate (OMP) to give uridine monophosphate(UMP) during pyrimidine biosynthesis. Uracil biosynthesis occurs in the cytosol.
To explain: The reason why the cells that are defective for mitochondrial import grow in the absence of uracil.
Concept introduction: Ura 3 gene is involved in pyrimidine biosynthesis. Ura 3 gene produces an enzyme called Orotidine 5'-phosphate decarboxylase. Orotidine 5'-phosphate decarboxylase catalyses the decarboxylation step of orotidine monophosphate (OMP) to give uridine monophosphate(UMP) during pyrimidine biosynthesis. Uracil biosynthesis occurs in the cytosol.
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Molecular Biology of the Cell (Sixth Edition)
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