To differentiate: The Sanger sequencing and high throughput sequencing. Introduction: The genome of an organism is defined as the whole genetic information that is inherited from one generation to the next generation. It includes the protein-coding genes, non-protein coding genes, regulatory genes, chloroplast DNA and mitochondrial DNA. The human genome consists of about 2.5 billion gens of which only 20,000 genes are protein-coding genes.

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Answer 1

Question

Chapter 12, Problem 1.1Q

Summary Introduction

To differentiate: The Sanger sequencing and high throughput sequencing.

Introduction: The genome of an organism is defined as the whole genetic information that is inherited from one generation to the next generation. It includes the protein-coding genes, non-protein coding genes, regulatory genes, chloroplast DNA and mitochondrial DNA. The human genome consists of about 2.5 billion gens of which only 20,000 genes are protein-coding genes.

Expert Solution & Answer

Explanation of Solution

The difference between the Sanger sequencing and high throughput sequencing is described as follows:

Characteristics	Sanger sequencing	High throughput sequencing
Template preparation	The direct DNA template is used.	The PCR technique is used to amplify the template.
Sequencing chemistry	Chain termination	Sequencing by amplification.
Fluoresce tags.	The fluoresce is tagged with deoxyribonucleotides.	The fluoresce is tagged with deoxyribonucleotides.
Rounds	It involves a single round of sequencing.	It involves multiple rounds of sequencing.
Number of base pairs.	It can sequence about 300 to 1000 bases.	It can sequence about a million bases.

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Answer 2

Question

Chapter 12, Problem 1.1Q

Summary Introduction

To differentiate: The Sanger sequencing and high throughput sequencing.

Introduction: The genome of an organism is defined as the whole genetic information that is inherited from one generation to the next generation. It includes the protein-coding genes, non-protein coding genes, regulatory genes, chloroplast DNA and mitochondrial DNA. The human genome consists of about 2.5 billion gens of which only 20,000 genes are protein-coding genes.

Expert Solution & Answer

Explanation of Solution

The difference between the Sanger sequencing and high throughput sequencing is described as follows:

Characteristics	Sanger sequencing	High throughput sequencing
Template preparation	The direct DNA template is used.	The PCR technique is used to amplify the template.
Sequencing chemistry	Chain termination	Sequencing by amplification.
Fluoresce tags.	The fluoresce is tagged with deoxyribonucleotides.	The fluoresce is tagged with deoxyribonucleotides.
Rounds	It involves a single round of sequencing.	It involves multiple rounds of sequencing.
Number of base pairs.	It can sequence about 300 to 1000 bases.	It can sequence about a million bases.

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Answer 3

Question

Chapter 12, Problem 1.1Q

Summary Introduction

To differentiate: The Sanger sequencing and high throughput sequencing.

Introduction: The genome of an organism is defined as the whole genetic information that is inherited from one generation to the next generation. It includes the protein-coding genes, non-protein coding genes, regulatory genes, chloroplast DNA and mitochondrial DNA. The human genome consists of about 2.5 billion gens of which only 20,000 genes are protein-coding genes.

Expert Solution & Answer

Explanation of Solution

The difference between the Sanger sequencing and high throughput sequencing is described as follows:

Characteristics	Sanger sequencing	High throughput sequencing
Template preparation	The direct DNA template is used.	The PCR technique is used to amplify the template.
Sequencing chemistry	Chain termination	Sequencing by amplification.
Fluoresce tags.	The fluoresce is tagged with deoxyribonucleotides.	The fluoresce is tagged with deoxyribonucleotides.
Rounds	It involves a single round of sequencing.	It involves multiple rounds of sequencing.
Number of base pairs.	It can sequence about 300 to 1000 bases.	It can sequence about a million bases.

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Answer 4

Question

Chapter 12, Problem 1.1Q

Summary Introduction

To differentiate: The Sanger sequencing and high throughput sequencing.

Introduction: The genome of an organism is defined as the whole genetic information that is inherited from one generation to the next generation. It includes the protein-coding genes, non-protein coding genes, regulatory genes, chloroplast DNA and mitochondrial DNA. The human genome consists of about 2.5 billion gens of which only 20,000 genes are protein-coding genes.

Expert Solution & Answer

Explanation of Solution

The difference between the Sanger sequencing and high throughput sequencing is described as follows:

Characteristics	Sanger sequencing	High throughput sequencing
Template preparation	The direct DNA template is used.	The PCR technique is used to amplify the template.
Sequencing chemistry	Chain termination	Sequencing by amplification.
Fluoresce tags.	The fluoresce is tagged with deoxyribonucleotides.	The fluoresce is tagged with deoxyribonucleotides.
Rounds	It involves a single round of sequencing.	It involves multiple rounds of sequencing.
Number of base pairs.	It can sequence about 300 to 1000 bases.	It can sequence about a million bases.

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Answer 5

Chapter 12, Problem 1.1Q

Summary Introduction

To differentiate: The Sanger sequencing and high throughput sequencing.

Introduction: The genome of an organism is defined as the whole genetic information that is inherited from one generation to the next generation. It includes the protein-coding genes, non-protein coding genes, regulatory genes, chloroplast DNA and mitochondrial DNA. The human genome consists of about 2.5 billion gens of which only 20,000 genes are protein-coding genes.

Expert Solution & Answer

Explanation of Solution

The difference between the Sanger sequencing and high throughput sequencing is described as follows:

Characteristics	Sanger sequencing	High throughput sequencing
Template preparation	The direct DNA template is used.	The PCR technique is used to amplify the template.
Sequencing chemistry	Chain termination	Sequencing by amplification.
Fluoresce tags.	The fluoresce is tagged with deoxyribonucleotides.	The fluoresce is tagged with deoxyribonucleotides.
Rounds	It involves a single round of sequencing.	It involves multiple rounds of sequencing.
Number of base pairs.	It can sequence about 300 to 1000 bases.	It can sequence about a million bases.

Answer 6

Chapter 12, Problem 1.1Q

Summary Introduction

To differentiate: The Sanger sequencing and high throughput sequencing.

Introduction: The genome of an organism is defined as the whole genetic information that is inherited from one generation to the next generation. It includes the protein-coding genes, non-protein coding genes, regulatory genes, chloroplast DNA and mitochondrial DNA. The human genome consists of about 2.5 billion gens of which only 20,000 genes are protein-coding genes.

Expert Solution & Answer

Explanation of Solution

The difference between the Sanger sequencing and high throughput sequencing is described as follows:

Characteristics	Sanger sequencing	High throughput sequencing
Template preparation	The direct DNA template is used.	The PCR technique is used to amplify the template.
Sequencing chemistry	Chain termination	Sequencing by amplification.
Fluoresce tags.	The fluoresce is tagged with deoxyribonucleotides.	The fluoresce is tagged with deoxyribonucleotides.
Rounds	It involves a single round of sequencing.	It involves multiple rounds of sequencing.
Number of base pairs.	It can sequence about 300 to 1000 bases.	It can sequence about a million bases.

Answer 7

Chapter 12, Problem 1.1Q

Summary Introduction

To differentiate: The Sanger sequencing and high throughput sequencing.

Introduction: The genome of an organism is defined as the whole genetic information that is inherited from one generation to the next generation. It includes the protein-coding genes, non-protein coding genes, regulatory genes, chloroplast DNA and mitochondrial DNA. The human genome consists of about 2.5 billion gens of which only 20,000 genes are protein-coding genes.

Answer 8

Expert Solution & Answer

Explanation of Solution

The difference between the Sanger sequencing and high throughput sequencing is described as follows:

Characteristics	Sanger sequencing	High throughput sequencing
Template preparation	The direct DNA template is used.	The PCR technique is used to amplify the template.
Sequencing chemistry	Chain termination	Sequencing by amplification.
Fluoresce tags.	The fluoresce is tagged with deoxyribonucleotides.	The fluoresce is tagged with deoxyribonucleotides.
Rounds	It involves a single round of sequencing.	It involves multiple rounds of sequencing.
Number of base pairs.	It can sequence about 300 to 1000 bases.	It can sequence about a million bases.