Brock Biology of Microbiology - Modern MasteringBiology
15th Edition
ISBN: 9780134602325
Author: MADIGAN
Publisher: PEARSON
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Chapter 12, Problem 4AQ
Describe how you could recode Escherichia coli to produce novel proteins containing more than the standard 22 amino acids.
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Chapter 12 Solutions
Brock Biology of Microbiology - Modern MasteringBiology
Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQCh. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQCh. 12.2 - Prob. 3MQCh. 12.2 - Prob. 1CRCh. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...
Ch. 12.3 - Prob. 3MQCh. 12.3 - Prob. 1CRCh. 12.4 - How can site-directed mutagenesis be useful to...Ch. 12.4 - What is used to alter more than a few base pairs...Ch. 12.4 - What are knockout mutations?Ch. 12.4 - What does site-directed mutagenesis allow you to...Ch. 12.5 - What is a reporter gene? The product of which...Ch. 12.5 - Prob. 2MQCh. 12.5 - Describe two widely used reporter genes.Ch. 12.6 - Prob. 1MQCh. 12.6 - Prob. 2MQCh. 12.6 - Prob. 3MQCh. 12.6 - Prob. 1CRCh. 12.7 - Prob. 1MQCh. 12.7 - Give an example of a genetically modified plant...Ch. 12.7 - How have transgenic salmon been engineered to...Ch. 12.7 - What is the Ti plasmid and how has it been of use...Ch. 12.8 - Explain why recombinant vaccines might be safer...Ch. 12.8 - Prob. 2MQCh. 12.8 - Prob. 3MQCh. 12.8 - What is a subunit vaccine and why are subunit...Ch. 12.9 - Explain why metagenomic cloning gives large...Ch. 12.9 - What types of environments are often sampled to...Ch. 12.9 - Prob. 3MQCh. 12.9 - How has metagenomics been used to find novel...Ch. 12.10 - How has Caldicellulosiruptor been modified to...Ch. 12.10 - Prob. 2MQCh. 12.10 - What has been the limiting factor in engineering...Ch. 12.10 - Prob. 1CRCh. 12.11 - What are biobricks?Ch. 12.11 - Prob. 2MQCh. 12.11 - How was Escherichia coli modified to produce a...Ch. 12.11 - Prob. 1CRCh. 12.12 - Prob. 1MQCh. 12.12 - Prob. 2MQCh. 12.12 - How is recombinant DNA inserted into a genome...Ch. 12.12 - How has the CRISPR editing technology been applied...Ch. 12.13 - Prob. 1MQCh. 12.13 - How can a tRNA be engineered to encode for a...Ch. 12.13 - Prob. 3MQCh. 12.13 - What are some mechanisms for controlling a...Ch. 12 - Suppose you have just determined the DNA base...Ch. 12 - Prob. 2AQCh. 12 - Prob. 3AQCh. 12 - Describe how you could recode Escherichia coli to...
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- Using the “target DNA” sequence provided in the attached image, what sizes of inserts can be prepared using HindIII digestion? Which one contains the coding sequence?arrow_forwardWhen developing the zymogram using a destaining solution, a researcher observed wedge shaped bands with protein. What could be the possible reason for such an observation?arrow_forwardDescribe the chemotaxis system in E. coliarrow_forward
- From what other environments might E. coli bacteriophages be readily isolated?arrow_forwardHow would restriction enzymes play a role in having an organismproduce a protein that it normally doesn’t make?arrow_forwardfrederic griffith used the word transformation to describe the changes in bacteria that he observed. which is the most useful definition of transformation?arrow_forward
- Give three reasons why liposomes/nanoparticles are attractive drug delivery system for recombinant protein preparations?arrow_forwardAn E. coli colony grew on minimal medium supplemented with arginine and leucine. However, bacteria from this colony are unable to grow and form colonies on minimal medium supplemented with arginine and methionine. What is the genotype of the bacteria in this E. coli colony?arrow_forwardWrite a result paragraph of the isolation and purification of bacteriophage with E. coli Top10 with the following results: At 10^-1 to 10^-3 no plaques were formed. At 10^-4 too numerous plaques were formed. At 10^-5, 10^-6 and 10^-7 countable plates of 22, 7 and 1 respectively. At 10^-8 and 10^-9 no plaques formed but present of host bacteria.arrow_forward
- When using bacteria to produce a particular protein, why is it usually better to use the cDNA version of the gene rather than the genomic DNA version?arrow_forwardIn order to transform a bacteria, the cell wall should be perforated either through physical or chemical means. true or false?arrow_forwardHow does pseudomurein resemble peptidoglycan? How do thetwo molecules differ?arrow_forward
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