Concept explainers
To determine:
The product and density of DNA after one and two rounds of replication if Meselson and Stahl had started their experiment with 14N light DNA and then transferred them into 15N heavy DNA medium.
Concept introduction:
Meselson and Stahl performed an experiment to prove that DNA (deoxyribonucleic acid) replicates in a semi-conservative manner. They first grew bacterial cells in 15N medium, so that the DNA of the bacterial cells would only contain pure 15N DNA. Afterwards, these cells were transferred into 14N medium.
After one round of replication, DNA was centrifuged to observe their density in a solution containing cesium chloride. The density of parental DNA (15N/15N) was found to be
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