Why it is necessary to pay attention to the reading frame while choosing where to insert the gene into the plasmid. Introduction: The reading frame is three consecutive bases in the nucleic acid , that is divided into consecutive, non-overlapping triplets. These consecutive, non-overlapping triplets are called codons. Each triplet encodes an amino acid.

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Answer 1

Question

Chapter 13, Problem 1QC

Summary Introduction

To determine: Why it is necessary to pay attention to the reading frame while choosing where to insert the gene into the plasmid.

Introduction: The reading frame is three consecutive bases in the nucleic acid, that is divided into consecutive, non-overlapping triplets. These consecutive, non-overlapping triplets are called codons. Each triplet encodes an amino acid.

Expert Solution & Answer

Explanation of Solution

The genes of interest are inserted next to the promoter in the plasmid. The open reading frame (shortly ORF) is a part of the reading frame, which is capable of being translated. ORFs are defined as the continuous stretch of codons present between the start and stop codons. When the translation is initiated, the ribosome selects a reading frame starting from initiation codon and then translates the selected reading frame until it reaches the stop codon. Thus, it is essential to pay attention to the reading frames for proper translation to occur.

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Answer 2

Question

Chapter 13, Problem 1QC

Summary Introduction

To determine: Why it is necessary to pay attention to the reading frame while choosing where to insert the gene into the plasmid.

Introduction: The reading frame is three consecutive bases in the nucleic acid, that is divided into consecutive, non-overlapping triplets. These consecutive, non-overlapping triplets are called codons. Each triplet encodes an amino acid.

Expert Solution & Answer

Explanation of Solution

The genes of interest are inserted next to the promoter in the plasmid. The open reading frame (shortly ORF) is a part of the reading frame, which is capable of being translated. ORFs are defined as the continuous stretch of codons present between the start and stop codons. When the translation is initiated, the ribosome selects a reading frame starting from initiation codon and then translates the selected reading frame until it reaches the stop codon. Thus, it is essential to pay attention to the reading frames for proper translation to occur.

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Answer 3

Question

Chapter 13, Problem 1QC

Summary Introduction

To determine: Why it is necessary to pay attention to the reading frame while choosing where to insert the gene into the plasmid.

Introduction: The reading frame is three consecutive bases in the nucleic acid, that is divided into consecutive, non-overlapping triplets. These consecutive, non-overlapping triplets are called codons. Each triplet encodes an amino acid.

Expert Solution & Answer

Explanation of Solution

The genes of interest are inserted next to the promoter in the plasmid. The open reading frame (shortly ORF) is a part of the reading frame, which is capable of being translated. ORFs are defined as the continuous stretch of codons present between the start and stop codons. When the translation is initiated, the ribosome selects a reading frame starting from initiation codon and then translates the selected reading frame until it reaches the stop codon. Thus, it is essential to pay attention to the reading frames for proper translation to occur.

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Answer 4

Question

Chapter 13, Problem 1QC

Summary Introduction

To determine: Why it is necessary to pay attention to the reading frame while choosing where to insert the gene into the plasmid.

Introduction: The reading frame is three consecutive bases in the nucleic acid, that is divided into consecutive, non-overlapping triplets. These consecutive, non-overlapping triplets are called codons. Each triplet encodes an amino acid.

Expert Solution & Answer

Explanation of Solution

The genes of interest are inserted next to the promoter in the plasmid. The open reading frame (shortly ORF) is a part of the reading frame, which is capable of being translated. ORFs are defined as the continuous stretch of codons present between the start and stop codons. When the translation is initiated, the ribosome selects a reading frame starting from initiation codon and then translates the selected reading frame until it reaches the stop codon. Thus, it is essential to pay attention to the reading frames for proper translation to occur.

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Answer 5

Chapter 13, Problem 1QC

Summary Introduction

To determine: Why it is necessary to pay attention to the reading frame while choosing where to insert the gene into the plasmid.

Introduction: The reading frame is three consecutive bases in the nucleic acid, that is divided into consecutive, non-overlapping triplets. These consecutive, non-overlapping triplets are called codons. Each triplet encodes an amino acid.

Expert Solution & Answer

Explanation of Solution

The genes of interest are inserted next to the promoter in the plasmid. The open reading frame (shortly ORF) is a part of the reading frame, which is capable of being translated. ORFs are defined as the continuous stretch of codons present between the start and stop codons. When the translation is initiated, the ribosome selects a reading frame starting from initiation codon and then translates the selected reading frame until it reaches the stop codon. Thus, it is essential to pay attention to the reading frames for proper translation to occur.

Answer 6

Chapter 13, Problem 1QC

Summary Introduction

To determine: Why it is necessary to pay attention to the reading frame while choosing where to insert the gene into the plasmid.

Introduction: The reading frame is three consecutive bases in the nucleic acid, that is divided into consecutive, non-overlapping triplets. These consecutive, non-overlapping triplets are called codons. Each triplet encodes an amino acid.

Expert Solution & Answer

Explanation of Solution

The genes of interest are inserted next to the promoter in the plasmid. The open reading frame (shortly ORF) is a part of the reading frame, which is capable of being translated. ORFs are defined as the continuous stretch of codons present between the start and stop codons. When the translation is initiated, the ribosome selects a reading frame starting from initiation codon and then translates the selected reading frame until it reaches the stop codon. Thus, it is essential to pay attention to the reading frames for proper translation to occur.

Answer 7

Chapter 13, Problem 1QC

Summary Introduction

To determine: Why it is necessary to pay attention to the reading frame while choosing where to insert the gene into the plasmid.

Introduction: The reading frame is three consecutive bases in the nucleic acid, that is divided into consecutive, non-overlapping triplets. These consecutive, non-overlapping triplets are called codons. Each triplet encodes an amino acid.

Answer 8

Expert Solution & Answer

Explanation of Solution

The genes of interest are inserted next to the promoter in the plasmid. The open reading frame (shortly ORF) is a part of the reading frame, which is capable of being translated. ORFs are defined as the continuous stretch of codons present between the start and stop codons. When the translation is initiated, the ribosome selects a reading frame starting from initiation codon and then translates the selected reading frame until it reaches the stop codon. Thus, it is essential to pay attention to the reading frames for proper translation to occur.