PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 2818440045677
Author: WILLEY
Publisher: MCG
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Textbook Question
Chapter 14, Problem 2AL
Describe the
- a. A strain with a mutation in the gene encoding the lac repressor; the mutant repressor cannot bind allolactose.
- b. A strain with a mutation in the gene encoding CAP; the mutant form of CAP binds but cannot release cAMP.
- c. A strain in which the Shine-Dalgarno sequence has been deleted from the gene encoding adenyl cyclase.
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Chapter 14 Solutions
PRESCOTT'S MICROBIOLOGY
Ch. 14.2 - MICRO INQUIRY In what way is on inducer molecule...Ch. 14.2 - MICRO INQUIRY Is allolactose a corepressor or...Ch. 14.2 - Retrieve, Infer, Apply Many genes and operons are...Ch. 14.2 - Prob. 2CCCh. 14.2 - Prob. 3CCCh. 14.3 - MICRO INQUIRY How does this attenuation respond to...Ch. 14.4 - MICRO INQUIRY How does inhibition of translation...Ch. 14.4 - Prob. 1CCCh. 14.4 - Prob. 2CCCh. 14.4 - Prob. 3CC
Ch. 14.4 - Retrieve, Infer, Apply How are translational...Ch. 14.4 - Prob. 5CCCh. 14.5 - MICRO INQUIRY Relative to each promoter, where...Ch. 14.5 - MICRO INQUIRY For what other compounds would you...Ch. 14.5 - Prob. 1CCCh. 14.5 - Retrieve, Infer, Apply What is diauxic growth?...Ch. 14.5 - Retrieve, Infer, Apply Describe the events that...Ch. 14.5 - Retrieve, Infer, Apply E. coli has two phosphate...Ch. 14.5 - Prob. 5CCCh. 14.6 - Prob. 1MICh. 14.6 - MICRO INQUIRY Why does V. harveyi make three...Ch. 14.6 - Prob. 3MICh. 14.6 - Prob. 1CCCh. 14.6 - Prob. 2CCCh. 14.6 - Prob. 3CCCh. 14.6 - Retrieve, Infer, Apply Why might bacteria use...Ch. 14.6 - Prob. 5CCCh. 14.6 - Prob. 6CCCh. 14 - Prob. 1RCCh. 14 - Prob. 2RCCh. 14 - Prob. 3RCCh. 14 - Prob. 4RCCh. 14 - Attenuation affects anabolic pathways, whereas...Ch. 14 - Describe the phenotype of the following E. coli...Ch. 14 - Prob. 3ALCh. 14 - What would be the phenotype of a B. subtilis...Ch. 14 - Propose a mechanism by which a cell might sense...Ch. 14 - Prob. 6ALCh. 14 - Prob. 7ALCh. 14 - Prob. 8ALCh. 14 - Prob. 9AL
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- The above answer did not address the question in the sense that the answer did not indicate which protein involved in the pathways could be measured in the nucleus of cells and be confident in the results? I reiterate the question as follows: You want to do a single Western blot to measure changes in signaling activity, regardless of which pathway is being activated. For which protein involved in these pathways could you measure the levels in the nucleus of cells and be confident in your results? Why?arrow_forwardExplain Pleasearrow_forwarda. Would you expect a cell to continue or to stopdividing at a nonpermissive high temperature if itis a temperature-sensitive Ras mutant whose protein product is fixed in the GTP-bound form atnonpermissive temperature?b. What would you expect if you had a temperaturesensitive mutant in which the Ras protein staysin the GDP-bound form at high temperature?arrow_forward
- If the lacl gene is mutated so that the repressor protein no longer binds to lactose, what will be the effect on the expression of B-galactosidase in lactose's presence and absence? Explain. If the promoter for lacl is mutated so that the expression of the repressor increases, what will be the effect on the expression of B-galactosidase in the presence and absence of lactose? Explain. D. (Extremely tricky question!) Describe the behavior of the lac operon assuming that the lacl gene has been mutated so that the repressor now binds to DNA in the presence of lactose but cannot bind to DNA in the absence of lactose.arrow_forwardPlease explainarrow_forwardConsider the mechanism of the enzyme RNase: What would happen to the Km (i.e., would it increase, decrease, or stay the same) if the his12 was mutated to a lysine? Explain. What would happen to the Kcat (i.e., would it increase, decrease, or stay the same) if the his12 was mutated to a valine? Explain.arrow_forward
- The graph below shows a growth curve of E. coli growing in a defined medium containing equal concentration of glucose and lactose. On the y-axis optical density provides a measure of cell number, higher optical density indicates greater cell number. OD (optical density) Time Based on your knowledge regarding regulation of the lac operon, the best explanation of these results is that... A. The bacteria are utilizing both sugars simultaneously. B. At early time points, inhibition of the lac repressor allows for lactose metabolism. C. Cells constitutively express enzymes needed for sugar metabolism, allowing for continuous cell growth. D. Glucose activates CAP, allowing for cell growth at intermediate time points. E. At later time points, high cAMP allows for an increase in beta-galactosidase expression.arrow_forwardClary Fray used the pET vector system to express her prokaryotic amylase enzyme. She added peptone into her culture broth of BL21(DE3) Escherichia coli strain to induce protein expression. At the end of the experiment, she discovered that her protein was not expressed. She repeated three more times but her protein of interest was still not produced. (i) (ii) (iii) (iv) (v) Explain the reason why Clary failed to obtain her protein of interest and suggest a solution to troubleshoot this problem. Clary plans to express her protein along with a polyhistidine-tag. Explain the importance of His-tag in protein work. Is DH5a Escherichia coli suitable to propagate the plasmid before protein expression? Besides heat shock method, elaborate another procedure Clary could utilize to transform the recombinant pET vector into the host cell. If her supervisor instructs her to express a gene from gold fish (Carassius auratus), is the expression system she is using now suitable for this experiment?…arrow_forwardAnswer question 3arrow_forward
- Describe the mechanism by which cAMP receptorprotein (CRP), the regulatory protein for catabolite repression, functions. Use the lactose operon as an example.arrow_forwardSuppose that E. coli sustains a mutation in its gene for the lac operon repressor such that the repressor is ineffective. What effect would this have on the bacterium’s ability to catabolize lactose? Would the mutant strain have an advantage over wild-type cells? Explain your answer.arrow_forwardConsider the following simple regulatory pathways. Assume the full pathway is shown. A- E- B- F- C- G- D- 1 H- A You identify several null mutations (a complete deletion of the gene). For each mutant (ind with a - sign), determine whether the final product (I, J, K or L) is inducible, uninducible, or constitutive. 2 B 3 C 4 D inducible inducible constitutive uninducible constitutive inducible inducible E uninducible F G H > > >arrow_forward
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