2 SEM ACC W/RAVEN CARDED
12th Edition
ISBN: 9781264439218
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 14, Problem 4A
Summary Introduction
Introduction:
Leading strand of DNA is that strand of DNA that is synthesized continuously. It is the strand in which the replication takes place in 5’ to 3’ direction or in the direction of the fork. Lagging strand is that strand in which the replication occurs discontinuously. In this strand the synthesis occurs with the help of short DNA fragments called the Okazaki fragments.
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Which of the following types of DNA damage would be hardest to repair using the DNA repair pathways?A. Complete removal of three nucleotides in the middle of one strand.B. A covalent bond between a base on one strand and a base on the complementary strand.C. Incorporation of a sugar other than deoxyribose into one strand.D. Covalent attachment of a short polypeptide to a single base.E. A covalent bond between a base and a deoxyribose on the same strand.
Please explain why it's B
The function of DNA ligase is to:
a. Catalyze formation of phosphodiester bonds between adjacent nucleotides
b. Catalyze formation of hydrogen bonds between adjacent nucleotides
c. Keep single strands of DNA apart during replication
d. Facilitate base pairing between single stranded molecules in DNA
e. Both a. and d. are correct
Indicate whether each of the following statementsrelating to aspects of DNA replication is true or false.a. The lagging strand grows in the same direction as thereplication fork moves.b. Growth of the leading strand involves the productionof Okazaki fragments.c. Lagging strands always involve “daughter” DNAsegments, and leading strands always involve“parent” DNA segments.d. The enzyme DNA ligase effects the unwinding of aDNA double helix.
Chapter 14 Solutions
2 SEM ACC W/RAVEN CARDED
Ch. 14.1 - Describe the experiments of Griffith and Avery.Ch. 14.1 - Evaluate the evidence for DNA as genetic material.Ch. 14.2 - Explain how the WatsonCrick structure rationalized...Ch. 14.2 - Prob. 2LOCh. 14.3 - Prob. 1LOCh. 14.3 - Prob. 2LOCh. 14.4 - Prob. 1LOCh. 14.4 - Prob. 2LOCh. 14.4 - Diagram the functions found at the replication...Ch. 14.5 - Compare eukaryotic replication with prokaryotic.
Ch. 14.5 - Prob. 2LOCh. 14.5 - Prob. 3LOCh. 14.6 - Prob. 1LOCh. 14.6 - Prob. 2LOCh. 14 - Prob. 1DACh. 14 - Prob. 2DACh. 14 - Prob. 1IQCh. 14 - Prob. 2IQCh. 14 - How does the structure of eukaryotic genomes...Ch. 14 - Prob. 4IQCh. 14 - Prob. 1UCh. 14 - Which of the following is NOT a component of DNA?...Ch. 14 - Chargaff studied the composition of DNA from...Ch. 14 - The bonds that hold two complementary strands of...Ch. 14 - Prob. 5UCh. 14 - Prob. 6UCh. 14 - Which of the following is NOT pan of the...Ch. 14 - If one strand of a DNA is 5 ATCGTTAAGCGAGTCA 3,...Ch. 14 - Hershey and Chase used radioactive phosphorus and...Ch. 14 - The Meselson and Stahl experiment used a density...Ch. 14 - Prob. 4ACh. 14 - If the activity of DNA ligase was removed from...Ch. 14 - Successful DNA synthesis requires all of the...Ch. 14 - The synthesis of telomeres a. uses DNA polymerase,...Ch. 14 - When mutations that affected DNA replication were...Ch. 14 - Prob. 1SCh. 14 - In the Meselson-Stahl experiment, a control...Ch. 14 - Enzyme function is critically important for the...
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- Place the following steps of DNA replication and repair in the correct order by numbering them from 1 to 5. a. A template strand begins to be replicated. b. If the incorrect base is not identified and replaced, it remains as a point mutation in the DNA. c. DNA polymerase identifies and replaces most incorrect bases with the correct base, complementary to the base on the template strand. d. An incorrect base is added to the growing strand of DNA. e. Proteins identify and replace any incorrect bases missed by DNA polymerase.arrow_forward(1) DNA polymerase can only add nucleotides to an available 3' end. Why? a. The enzyme is attracted to the negatively charged phosphate group. b. The phosphate group at the 5' end interferes physically and/or electrically with the elongation process. c. The lagging strand needs to be synthesized more slowly. d. Okazaki fragments are critical to the formation of identical copies of DNA. e. No one knows.arrow_forwardWhich of the following statements is not true? Explain why. A. A DNA strand can serve as a template strand on many occasions. B. Following semiconservative DNA replication, one strand is a newly made daughter strand and the other strand is a parental strand. C. A DNA double helix may contain two strands of DNA that were made at the same time. D. A DNA double helix obeys the AT/GC rule. E. A DNA double helix could contain one strand that is 10 generations older than its complementary strand.arrow_forward
- During DNA replication, short RNA primers are made by the Primase. Why? a. To provide a 3'-OH so DNA polymerase can begin DNA synthesis. b. To recruit single stranded binding proteins to the correct location. c. To identify the termination sequence for DNA polymerase during DNA synthesis. d. To provide a 3'-OH so RNA polymerase can begin DNA synthesis. e. To identify the origin of replication to recruit the origin replication complex to the correct genomic location.arrow_forwardWhich of the following statements is TRUE concerning the synthesis of the leading and lagging strands of DNA in prokaryotic cells? a. O b. The leading strand is synthesized by one polymerase III continuously, and the lagging strand is synthesized by several molecules of DNA polymerase III. d. The leading and lagging strands are synthesized at the same time by the one DNA polymerase I. O c. The leading and lagging strands are synthesized at the same time by the one DNA polymerase III. The leading strand is synthesized by one polymerase III, and the lagging strand is synthesized by DNA polymerase I.arrow_forwardFor the statements below, indicate whether the statement applies to the leading strand, or to the lagging strand, or to both. a. synthesized in the 5'→3' direction b. synthesized continuously c. require(s) DNA ligase to join fragments d. described as a daughter strand e. synthesized in the same direction as the movement of the replication fork f. DNA polymerase is the enzyme involved in forming this polynucleotide.arrow_forward
- Determine whether each statement isTRUE or FALSE.a. In eukaryotic chromosomes, DNA replication begins at a single point in the chromosome and proceeds in two directions.b. The bonds between the sugars and phosphates are broken during DNA replication.c. The elongation of the leading strand during DNA synthesis progresses away from the replication fork.d. Each DNA molecule resulting from replication has one original strand and one new strand.e. The difference in how the leading and lagging strands of DNA molecules are synthesized is due to DNA polymerase adding new nucleotides only to the 3’ end of a growing strand, and the strands are anti-parallel.arrow_forwardHow would nucleotide excision repair be affected if one of the followingproteins was missing? Describe the condition of the DNAif the repair was attempted in the absence of the protein.A. UvrAB. UvrCC. UvrDD. DNA polymerasearrow_forwardEnzyme function is critically important for the proper replication of DNA. Predict the consequence of a loss of function for each of the following enzymes. a. DNA gyrase b. DNA polymerase III c. DNA ligase d. DNA polymerase Iarrow_forward
- a. unwinds the DNA helix b. stabilizes and stops the two strands from annealing (rebinding with each other) c. recoils the DNA d. cleaves both strands of DNA to relieve tension at supercoils e. places RNA primers at their proper location on the template strands f. acts as starting points for DNA polymerase g. adds DNA nucleotides to form new DNA strands h. forms phosphodiester bonds to join Okazaki fragments Esc 1. single-strand binding protein 2. helicase 3. DNA ligase 4. RNA primer 5. gyrase 6. DNA polymerase 53°F Cloudy 3 Q Search $ F5 FRarrow_forwardThe problem of synthesizing the lagging strand, in the sense that DNA synthesis can only occur in one direction, is manifested inA. creation of primers every so often as more of the template strand isuntwisted.B. formation of shorter DNA strands called Okazaki fragments.C. the need for the enzyme ligase to link short DNA strands together.D. All of the above choices are correct.arrow_forwardThe antiparallel nature of DNA refers to a. its charged phosphate groups. b. the pairing of bases on one strand with bases on the other strand. c. the formation of hydrogen bonds between bases from opposite strands. d. the opposite direction of the two strands of nucleotides.arrow_forward
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