Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN: 9781133893943
Author: ESTRIDGE
Publisher: Cengage
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With the aid of diagrams, explain the processes and principles of each of the following
methods:
(a) Gel filtration chromatography on Sephadex G50
b.SDS-PAGE
Other than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.
Aside from application of heat, name other methods of sterilization and give examples of materials sterilized in each method
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- Alternative name for Moist-heat sterilization Select one: Oven sterilization Autoclaving Ionizing radiationarrow_forwardAnswer the ff with not more than two sentences: Why should Erlenmeyer flasks hold not more than 60% of their capacity? When can gelatin be used in culture media preparation? How does cotton plug prevent contamination of the medium? Why should caps of glassware be loosened first before sterilization?arrow_forwardIn sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperarrow_forward
- Make illustrated diagrams of stepwise procedures for the performance of the following tasks. 2. Sterilization of liquid culture media for platingarrow_forwardGive the advantages and disadvantages of the following dosage forms in sentence forms: ○ Tablets○ Capsules○ Syrups○ Suppositories○ Emulsionsarrow_forwardWhat will pose an ethical issue in these procedures are performed - sterilization?arrow_forward
- Solid and liquid media are commonly prepared based on manufacturer’s instructions. If no instruction is provided on how the media should be sterilized, what would be the best option? METHOD:RATIONALE:arrow_forwardRequirements of Autoclave for sterilizationarrow_forwardDefine disinfection. Define sterility. Compare and contrast disinfection with sterility.arrow_forward
- What is the meaning of BSC or biological safety cabinet and why is it important and how is it works?arrow_forwardPlease explain why is 70% ethanol used as an effective disinfecting solution in labs? Why not 95% ethanol? Please include references.arrow_forwardWheeler JohnsonPrinciple:Reagents:Reaction:Substance Detected: MurexidePrinciple:Reagents:Reaction:Substance Detected: 10% NH4OH & 2% AgNO3Principle:Reagents:Reaction:Substance Detected:arrow_forward
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