GEN COMBO LOOSELEAF MICROBIOLOGY:A SYSTEMS APPROACH; CONNECT ACCESS CARD
5th Edition
ISBN: 9781260149364
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Question
Chapter 17.4, Problem 15AYP
Summary Introduction
To explain:
The use of PCR in microbial identification tests.
Concept introduction:
Polymerase chain reaction (PCR) was developed by Kary Mullis in 1985. It is a technique, which is used to amplify DNA (deoxyribonucleic acid). PCR can create millions of copies of desired DNA sequence from a small amount of DNA. PCR is almost similar to
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Chapter 17 Solutions
GEN COMBO LOOSELEAF MICROBIOLOGY:A SYSTEMS APPROACH; CONNECT ACCESS CARD
Ch. 17.1 - Prob. 1AYPCh. 17.1 - Prob. 2AYPCh. 17.1 - Prob. 3AYPCh. 17.1 - Prob. 4AYPCh. 17.2 - Summarize the most common methods used for direct...Ch. 17.2 - Prob. 6AYPCh. 17.2 - Prob. 7AYPCh. 17.3 - Prob. 8AYPCh. 17.3 - Differentiate between sensitivity and specificity.Ch. 17.3 - Explain how fluorescent antibodies can be used in...
Ch. 17.3 - Prob. 11AYPCh. 17.3 - Prob. 12AYPCh. 17.3 - Prob. 13AYPCh. 17.3 - Explain the difference between a direct and an...Ch. 17.4 - Prob. 15AYPCh. 17.4 - Prob. 16AYPCh. 17.4 - Prob. 17AYPCh. 17.5 - Prob. 18AYPCh. 17.5 - List the major advantages of microarray methods of...Ch. 17.5 - Summarize the benefits of using whole-genome...Ch. 17 - Prob. 1MCQCh. 17 - Prob. 2MCQCh. 17 - Prob. 3MCQCh. 17 - Prob. 4MCQCh. 17 - Prob. 5MCQCh. 17 - Prob. 6MCQCh. 17 - Prob. 7MCQCh. 17 - Prob. 8MCQCh. 17 - The Western blot test can be used to identify a....Ch. 17 - Prob. 10MCQCh. 17 - True-False Questions. If the statement is true,...Ch. 17 - Prob. 12TFCh. 17 - Prob. 13TFCh. 17 - Prob. 14TFCh. 17 - Prob. 15TFCh. 17 - Explain why specimens should be taken aseptically,...Ch. 17 - Prob. 2CTQCh. 17 - Explain which type of ELISA can be used to...Ch. 17 - Prob. 4CTQCh. 17 - Prob. 5CTQCh. 17 - Prob. 1VCCh. 17 - From this chapter, figure 17.14. Can you explain...Ch. 17 - Prob. 1CM
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- Define the advantage of using PCR for infectious disease diagnosis.arrow_forwardWhat is the first step that should be done in PCR testing?arrow_forwardExplain how PCR is used to detect the presence of pathogens in patient samples. Look up an example of where this technique is used routinely in diagnosis.arrow_forward
- Describe the applications of PCR in identification techniques.arrow_forwardComprehensively discuss the principle and applications of PCR. Discuss the quality criteria for nucleic acid isolates. Explain the use of UV spectrometry in nucleic acid purification.arrow_forwardPCR is a useful tool for early diagnosis of an infectious disease.Elaborate.arrow_forward
- Briefly describe the functions of the components of a typical PCR reaction.arrow_forwardExplain how PCR can be used to detect infectious agents in diagnoses of diseases.arrow_forwardDiscuss how DNA extraction of microbial DNA directly can be done from infected tissue through an optimized protocol for use in nanopore sequencing.arrow_forward
- Determining the genetic profile of a bacterium for its identification is a part of which step of the identification process?arrow_forwardDiscuss the uses of PCR and RT-PCR in human identity (e.g. paternity, forensics or criminal cases) as well as in viral or bacterial DNA/RNA analysis (i.e. Identification of SARS-COV-2 RNA coronavirus using RT-PCR).arrow_forwardDescribe the applications of PCR in identifi cation techniques.arrow_forward
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